Imaging of native transcription factors and histone phosphorylation at high resolution in live cells
Fluorescent labeling of endogenous proteins for live-cell imaging without exogenous expression of tagged proteins or genetic manipulations has not been routinely possible. We describe a simple versatile antibody-based imaging approach (VANIMA) for the precise localization and tracking of endogenous...
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Main Authors: | , , , , , , , , , , , , |
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Other Authors: | |
Format: | Article |
Language: | English |
Published: |
2018
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Subjects: | |
Online Access: | https://hdl.handle.net/10356/88771 http://hdl.handle.net/10220/44728 |
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Institution: | Nanyang Technological University |
Language: | English |