Imaging of native transcription factors and histone phosphorylation at high resolution in live cells

• Main Authors: Conic, Sascha, Desplancq, Dominique, Ferrand, Alexia, Fischer, Veronique, Heyer, Vincent, Reina San Martin, Bernardo, Pontabry, Julien, Oulad-Abdelghani, Mustapha, Babu N., Kishore, Wright, Graham D., Molina, Nacho, Weiss, Etienne, Tora, László
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2018
• Subjects: Transcription; Histone
• Online Access: https://hdl.handle.net/10356/88771; http://hdl.handle.net/10220/44728
• Institution: Nanyang Technological University

Fluorescent labeling of endogenous proteins for live-cell imaging without exogenous expression of tagged proteins or genetic manipulations has not been routinely possible. We describe a simple versatile antibody-based imaging approach (VANIMA) for the precise localization and tracking of endogenous...