On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity
Tissue adhesives based on polyamidoamine (PAMAM) dendrimer, grafted with UV-sensitive aryldiazirine (PAMAM-g-diazirine) are promising new candidates for light active adhesion on soft tissues. Diazirine carbene precursors form interfacial and intermolecular covalent crosslinks with tissues after UV l...
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sg-ntu-dr.10356-890332023-07-14T15:45:53Z On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity Gao, Feng Djordjevic, Ivan Pokholenko, Oleksandr Zhang, Haobo Zhang, Junying Steele, Terry W. J. School of Materials Science & Engineering Dendrimers PAMAM Tissue adhesives based on polyamidoamine (PAMAM) dendrimer, grafted with UV-sensitive aryldiazirine (PAMAM-g-diazirine) are promising new candidates for light active adhesion on soft tissues. Diazirine carbene precursors form interfacial and intermolecular covalent crosslinks with tissues after UV light activation that requires no premixing or inclusion of free radical initiators. However, primary amines on the PAMAM dendrimer surface present a potential risk due to their cytotoxic and immunological effects. PAMAM-g-diazirine formulations with cationic pendant amines converted into neutral amide groups were evaluated. In vitro toxicity is reduced by an order of magnitude upon amine capping while retaining bioadhesive properties. The in vivo immunological response to PAMAM-g-diazirine formulations was found to be optimal in comparison to standard poly(lactic-co-glycolic acid) (PLGA) thin films. MOE (Min. of Education, S’pore) Published version 2018-05-09T04:19:44Z 2019-12-06T17:16:23Z 2018-05-09T04:19:44Z 2019-12-06T17:16:23Z 2018 Journal Article Gao, F., Djordjevic, I., Pokholenko, O., Zhang, H., Zhang, J., & Steele, T. W. J. (2018). On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity. Molecules, 23(4), 796-. 1420-3049 https://hdl.handle.net/10356/89033 http://hdl.handle.net/10220/44763 10.3390/molecules23040796 en Molecules © 2018 by The Author(s). Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). 13 p. application/pdf |
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Dendrimers PAMAM Gao, Feng Djordjevic, Ivan Pokholenko, Oleksandr Zhang, Haobo Zhang, Junying Steele, Terry W. J. On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity |
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Tissue adhesives based on polyamidoamine (PAMAM) dendrimer, grafted with UV-sensitive aryldiazirine (PAMAM-g-diazirine) are promising new candidates for light active adhesion on soft tissues. Diazirine carbene precursors form interfacial and intermolecular covalent crosslinks with tissues after UV light activation that requires no premixing or inclusion of free radical initiators. However, primary amines on the PAMAM dendrimer surface present a potential risk due to their cytotoxic and immunological effects. PAMAM-g-diazirine formulations with cationic pendant amines converted into neutral amide groups were evaluated. In vitro toxicity is reduced by an order of magnitude upon amine capping while retaining bioadhesive properties. The in vivo immunological response to PAMAM-g-diazirine formulations was found to be optimal in comparison to standard poly(lactic-co-glycolic acid) (PLGA) thin films. |
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School of Materials Science & Engineering |
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School of Materials Science & Engineering Gao, Feng Djordjevic, Ivan Pokholenko, Oleksandr Zhang, Haobo Zhang, Junying Steele, Terry W. J. |
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Article |
author |
Gao, Feng Djordjevic, Ivan Pokholenko, Oleksandr Zhang, Haobo Zhang, Junying Steele, Terry W. J. |
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Gao, Feng |
title |
On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity |
title_short |
On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity |
title_full |
On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity |
title_fullStr |
On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity |
title_full_unstemmed |
On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity |
title_sort |
on-demand bioadhesive dendrimers with reduced cytotoxicity |
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2018 |
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https://hdl.handle.net/10356/89033 http://hdl.handle.net/10220/44763 |
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1772828980097843200 |