Effects of pupil filter patterns in line-scan focal modulation microscopy

Effects of pupil filter patterns in line-scan focal modulation microscopy

Line-scan focal modulation microscopy (LSFMM) is an emerging imaging technique that affords high imaging speed and good optical sectioning at the same time. We present a systematic investigation into optimal design of the pupil filter for LSFMM in an attempt to achieve the best performance in terms...

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Bibliographic Details
Main Authors: Shen, Shuhao, Pant, Shilpa, Chen, Rui, Chen, Nanguang
Other Authors: School of Electrical and Electronic Engineering
Format: Article
Language:English
Published: 2018
Subjects:
Fluorescence Microscopy
Line-scan
Online Access:https://hdl.handle.net/10356/89144
http://hdl.handle.net/10220/44817
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Institution: Nanyang Technological University
Language: English
Description
Summary:Line-scan focal modulation microscopy (LSFMM) is an emerging imaging technique that affords high imaging speed and good optical sectioning at the same time. We present a systematic investigation into optimal design of the pupil filter for LSFMM in an attempt to achieve the best performance in terms of spatial resolutions, optical sectioning, and modulation depth. Scalar diffraction theory was used to compute light propagation and distribution in the system and theoretical predictions on system performance, which were then compared with experimental results.

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