Effects of pupil filter patterns in line-scan focal modulation microscopy

Effects of pupil filter patterns in line-scan focal modulation microscopy

Line-scan focal modulation microscopy (LSFMM) is an emerging imaging technique that affords high imaging speed and good optical sectioning at the same time. We present a systematic investigation into optimal design of the pupil filter for LSFMM in an attempt to achieve the best performance in terms...

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Main Authors: Shen, Shuhao, Pant, Shilpa, Chen, Rui, Chen, Nanguang
Other Authors: School of Electrical and Electronic Engineering
Format: Article
Language:English
Published: 2018
Subjects:
Fluorescence Microscopy
Line-scan
Online Access:https://hdl.handle.net/10356/89144
http://hdl.handle.net/10220/44817
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-891442020-03-07T14:02:36Z Effects of pupil filter patterns in line-scan focal modulation microscopy Shen, Shuhao Pant, Shilpa Chen, Rui Chen, Nanguang School of Electrical and Electronic Engineering Fluorescence Microscopy Line-scan Line-scan focal modulation microscopy (LSFMM) is an emerging imaging technique that affords high imaging speed and good optical sectioning at the same time. We present a systematic investigation into optimal design of the pupil filter for LSFMM in an attempt to achieve the best performance in terms of spatial resolutions, optical sectioning, and modulation depth. Scalar diffraction theory was used to compute light propagation and distribution in the system and theoretical predictions on system performance, which were then compared with experimental results. NMRC (Natl Medical Research Council, S’pore) Published version 2018-05-17T04:32:18Z 2019-12-06T17:18:50Z 2018-05-17T04:32:18Z 2019-12-06T17:18:50Z 2018 Journal Article Shen, S., Pant, S., Chen, R., & Chen, N. (2018). Effects of pupil filter patterns in line-scan focal modulation microscopy. Journal of Biomedical Optics, 23(3), 036008-. 1083-3668 https://hdl.handle.net/10356/89144 http://hdl.handle.net/10220/44817 10.1117/1.JBO.23.3.036008 en Journal of Biomedical Optics © 2018 Society of Photo-optical Instrumentation Engineers. This paper was published in Journal of Biomedical Optics and is made available as an electronic reprint (preprint) with permission of Society of Photo-optical Instrumentation Engineers. The published version is available at [http://dx.doi.org/10.1117/1.JBO.23.3.036008]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. 6 p. application/pdf
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Fluorescence Microscopy
Line-scan
spellingShingle Fluorescence Microscopy
Line-scan
Shen, Shuhao
Pant, Shilpa
Chen, Rui
Chen, Nanguang
Effects of pupil filter patterns in line-scan focal modulation microscopy
description Line-scan focal modulation microscopy (LSFMM) is an emerging imaging technique that affords high imaging speed and good optical sectioning at the same time. We present a systematic investigation into optimal design of the pupil filter for LSFMM in an attempt to achieve the best performance in terms of spatial resolutions, optical sectioning, and modulation depth. Scalar diffraction theory was used to compute light propagation and distribution in the system and theoretical predictions on system performance, which were then compared with experimental results.
author2 School of Electrical and Electronic Engineering
author_facet School of Electrical and Electronic Engineering
Shen, Shuhao
Pant, Shilpa
Chen, Rui
Chen, Nanguang
format Article
author Shen, Shuhao
Pant, Shilpa
Chen, Rui
Chen, Nanguang
author_sort Shen, Shuhao
title Effects of pupil filter patterns in line-scan focal modulation microscopy
title_short Effects of pupil filter patterns in line-scan focal modulation microscopy
title_full Effects of pupil filter patterns in line-scan focal modulation microscopy
title_fullStr Effects of pupil filter patterns in line-scan focal modulation microscopy
title_full_unstemmed Effects of pupil filter patterns in line-scan focal modulation microscopy
title_sort effects of pupil filter patterns in line-scan focal modulation microscopy
publishDate 2018
url https://hdl.handle.net/10356/89144
http://hdl.handle.net/10220/44817
_version_ 1681044188671508480

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