Automated fluorescence intensity and gradient analysis enables detection of rare fluorescent mutant cells deep within the tissue of RaDR mice

• Main Authors: Wadduwage, Dushan N., Kay, Jennifer, Singh, Vijay Raj, Kiraly, Orsolya, Sukup-Jackson, Michelle R., Rajapakse, Jagath, Engelward, Bevin P., So, Peter T. C.
• Other Authors: School of Computer Science and Engineering
• Format: Article
• Language: English
• Published: 2018
• Subjects: Homologous Recombination; Fluorescent Mutant Cells; DRNTU::Engineering::Computer science and engineering
• Online Access: https://hdl.handle.net/10356/89695; http://hdl.handle.net/10220/46313
• Institution: Nanyang Technological University

Homologous recombination (HR) events are key drivers of cancer-promoting mutations, and the ability to visualize these events in situ provides important information regarding mutant cell type, location, and clonal expansion. We have previously created the Rosa26 Direct Repeat (RaDR) mouse model wher...