Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast
Actin cables, composed of actin filament bundles nucleated by formins, mediate intracellular transport for cell polarity establishment and maintenance. We previously observed that metaphase cells preferentially promote actin cable assembly through cyclin-dependent kinase 1 (Cdk1) activity. However,...
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sg-ntu-dr.10356-897972023-02-28T17:01:57Z Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast Miao, Yansong Han, Xuemei Zheng, Liangzhen Xie, Ying Mu, Yuguang Yates, John R. Drubin, David G. School of Chemical and Biomedical Engineering School of Biological Sciences Actin Cell Division Actin cables, composed of actin filament bundles nucleated by formins, mediate intracellular transport for cell polarity establishment and maintenance. We previously observed that metaphase cells preferentially promote actin cable assembly through cyclin-dependent kinase 1 (Cdk1) activity. However, the relevant metaphase Cdk1 targets were not known. Here we show that the highly conserved actin filament crosslinking protein fimbrin is a critical Cdk1 target for actin cable assembly regulation in budding yeast. Fimbrin is specifically phosphorylated on threonine 103 by the metaphase cyclin–Cdk1 complex, in vivo and in vitro. On the basis of conformational simulations, we suggest that this phosphorylation stabilizes fimbrin’s N-terminal domain, and modulates actin filament binding to regulate actin cable assembly and stability in cells. Overall, this work identifies fimbrin as a key target for cell cycle regulation of actin cable assembly in budding yeast, and suggests an underlying mechanism. Published version 2018-12-21T04:27:46Z 2019-12-06T17:33:43Z 2018-12-21T04:27:46Z 2019-12-06T17:33:43Z 2016 Journal Article Miao, Y., Han, X., Zheng, L., Xie, Y., Mu, Y., Yates, J. R., & Drubin, D. G. (2016). Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast. Nature Communications, 7,11265-. doi:10.1038/ncomms11265 https://hdl.handle.net/10356/89797 http://hdl.handle.net/10220/47163 10.1038/ncomms11265 27068241 en Nature Communications © 2016 The Author(s) (Published by Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. 12 p. application/pdf |
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Actin Cell Division Miao, Yansong Han, Xuemei Zheng, Liangzhen Xie, Ying Mu, Yuguang Yates, John R. Drubin, David G. Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast |
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Actin cables, composed of actin filament bundles nucleated by formins, mediate intracellular transport for cell polarity establishment and maintenance. We previously observed that metaphase cells preferentially promote actin cable assembly through cyclin-dependent kinase 1 (Cdk1) activity. However, the relevant metaphase Cdk1 targets were not known. Here we show that the highly conserved actin filament crosslinking protein fimbrin is a critical Cdk1 target for actin cable assembly regulation in budding yeast. Fimbrin is specifically phosphorylated on threonine 103 by the metaphase cyclin–Cdk1 complex, in vivo and in vitro. On the basis of conformational simulations, we suggest that this phosphorylation stabilizes fimbrin’s N-terminal domain, and modulates actin filament binding to regulate actin cable assembly and stability in cells. Overall, this work identifies fimbrin as a key target for cell cycle regulation of actin cable assembly in budding yeast, and suggests an underlying mechanism. |
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School of Chemical and Biomedical Engineering |
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School of Chemical and Biomedical Engineering Miao, Yansong Han, Xuemei Zheng, Liangzhen Xie, Ying Mu, Yuguang Yates, John R. Drubin, David G. |
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Article |
author |
Miao, Yansong Han, Xuemei Zheng, Liangzhen Xie, Ying Mu, Yuguang Yates, John R. Drubin, David G. |
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Miao, Yansong |
title |
Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast |
title_short |
Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast |
title_full |
Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast |
title_fullStr |
Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast |
title_full_unstemmed |
Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast |
title_sort |
fimbrin phosphorylation by metaphase cdk1 regulates actin cable dynamics in budding yeast |
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2018 |
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https://hdl.handle.net/10356/89797 http://hdl.handle.net/10220/47163 |
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1759854505689612288 |