Reactive metabolite-induced protein glutathionylation: a potentially novel mechanism underlying acetaminophen hepatotoxicity

• Main Authors: Chan, James Chun Yip, Soh, Alex Cheow Khoon, Kioh, Dorinda Yan Qin, Li, Jianguo, Verma, Chandra, Koh, Siew Kwan, Beuerman, Roger Wilmer, Zhou, Lei, Chan, Eric Chun Yong
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2018
• Subjects: Post-translational Modifications; Metabolomics; DRNTU::Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/89802; http://hdl.handle.net/10220/46390
• Institution: Nanyang Technological University

Although covalent protein binding is established as the pivotal event underpinning acetaminophen (APAP) toxicity, its mechanistic details remain unclear. In this study, we demonstrated that APAP induces widespread protein glutathionylation in a time-, dose- and bioactivation-dependent manner in Hepa...