Rapid EC50 determination of hydrophobic toxicants in continuous droplet biomicrofluidics

• Main Authors: Ortiz, Raphael, Stuckey, David C., Steele, Terry W. J.
• Other Authors: School of Materials Science & Engineering
• Format: Article
• Language: English
• Published: 2019
• Subjects: Droplet; Engineering::Materials; Microfluidic
• Online Access: https://hdl.handle.net/10356/91261; http://hdl.handle.net/10220/49445
• Institution: Nanyang Technological University

Droplet-based bioassays are attractive for increasing throughput while minimizing reagent consumption. However, the choice of continuous oil phase within the bioassay can substantially alter assay outcomes when hydrophobic reagents and analytes dynamically partition between the oil and aqueous phase...