An optogenetic approach for assessing formation of neuronal connections in a co-culture system

• Main Authors: Su, Colin T. E., Yoon, Su-In, Marcy, Guillaume, Chin, Eunice W. M., Augustine, George James, Goh, Eyleen Lay Keow
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2015
• Subjects: DRNTU::Science::Biological sciences::Human anatomy and physiology::Neurobiology
• Online Access: https://hdl.handle.net/10356/93695; http://hdl.handle.net/10220/38336
• Institution: Nanyang Technological University

Here we describe a protocol to generate a co-culture consisting of 2 different neuronal populations. Induced pluripotent stem cells (iPSCs) are reprogrammed from human fibroblasts using episomal vectors. Colonies of iPSCs can be observed 30 days after initiation of fibroblast reprogramming. Pluripot...