An optogenetic approach for assessing formation of neuronal connections in a co-culture system

Here we describe a protocol to generate a co-culture consisting of 2 different neuronal populations. Induced pluripotent stem cells (iPSCs) are reprogrammed from human fibroblasts using episomal vectors. Colonies of iPSCs can be observed 30 days after initiation of fibroblast reprogramming. Pluripot...

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Bibliographic Details
Main Authors: Su, Colin T. E., Yoon, Su-In, Marcy, Guillaume, Chin, Eunice W. M., Augustine, George James, Goh, Eyleen Lay Keow
Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
Format: Article
Language:English
Published: 2015
Subjects:
Online Access:https://hdl.handle.net/10356/93695
http://hdl.handle.net/10220/38336
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Institution: Nanyang Technological University
Language: English
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