Sorting catalytically active polymersome nanoreactors by flow cytometry

Flow cytometry is a powerful technique for high-throughput,...

Full description

Saved in:
Bibliographic Details
Main Authors: Nallani, Madhavan, Woestenenk, Rob, Hans-Peter M. de Hoog, Dongen, Stijn F. M. van, Boezeman, Jan, Cornelissen, Jeroen J. L. M., Nolte, Roeland J. M., Hest, Jan C. M. van.
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2011
Subjects:
Online Access:https://hdl.handle.net/10356/93845
http://hdl.handle.net/10220/7282
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-93845
record_format dspace
spelling sg-ntu-dr.10356-938452020-06-01T10:26:30Z Sorting catalytically active polymersome nanoreactors by flow cytometry Nallani, Madhavan Woestenenk, Rob Hans-Peter M. de Hoog Dongen, Stijn F. M. van Boezeman, Jan Cornelissen, Jeroen J. L. M. Nolte, Roeland J. M. Hest, Jan C. M. van. School of Materials Science & Engineering DRNTU::Engineering::Materials::Biomaterials Flow cytometry is a powerful technique for high-throughput, fluorescence-activated screening and sorting of cells (FACS). This methodology has been extended by Griffiths to the screening of water-in-oil microdroplets filled with an in vitro protein expression system.[1–6] The catalytic gene product was detected by the transformation of a co-encapsulated profluorescent substrate into a fluorescent product. Here we report a strategy that involves a versatile one-step preparation procedure of enzyme filled porous and stable polymeric capsules (polymersomes). Since the pores of the capsules are small enough to keep enzymes in, whereas these are sufficiently large to allow (profluorescent) substrates to enter, enzyme activity screening can be performed by the build-up of fluorescence, followed by FACS. To prevent the substrate from diffusing out of the capsules, a trapping agent was added inside the capsule. With this technology we were able to separate enzymatically active polymersomes from non-filled or non-active polymersomes. 2011-10-17T03:26:29Z 2019-12-06T18:46:32Z 2011-10-17T03:26:29Z 2019-12-06T18:46:32Z 2008 2008 Journal Article Nallani, M., Woestenenk, R., Hoog, H. P. M. D., Dongen, S. F. M. V., Boezeman, J., Cornelissen, J. J. L. M., et al. (2009). Sorting Catalytically Active Polymersome Nanoreactors by Flow Cytometry. Small, 5(10), 1138-1143. 1613-6810 https://hdl.handle.net/10356/93845 http://hdl.handle.net/10220/7282 10.1002/smll.200801204 155293 en Small © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. 6 p.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Engineering::Materials::Biomaterials
spellingShingle DRNTU::Engineering::Materials::Biomaterials
Nallani, Madhavan
Woestenenk, Rob
Hans-Peter M. de Hoog
Dongen, Stijn F. M. van
Boezeman, Jan
Cornelissen, Jeroen J. L. M.
Nolte, Roeland J. M.
Hest, Jan C. M. van.
Sorting catalytically active polymersome nanoreactors by flow cytometry
author2 School of Materials Science & Engineering
author_facet School of Materials Science & Engineering
Nallani, Madhavan
Woestenenk, Rob
Hans-Peter M. de Hoog
Dongen, Stijn F. M. van
Boezeman, Jan
Cornelissen, Jeroen J. L. M.
Nolte, Roeland J. M.
Hest, Jan C. M. van.
format Article
author Nallani, Madhavan
Woestenenk, Rob
Hans-Peter M. de Hoog
Dongen, Stijn F. M. van
Boezeman, Jan
Cornelissen, Jeroen J. L. M.
Nolte, Roeland J. M.
Hest, Jan C. M. van.
author_sort Nallani, Madhavan
title Sorting catalytically active polymersome nanoreactors by flow cytometry
title_short Sorting catalytically active polymersome nanoreactors by flow cytometry
title_full Sorting catalytically active polymersome nanoreactors by flow cytometry
title_fullStr Sorting catalytically active polymersome nanoreactors by flow cytometry
title_full_unstemmed Sorting catalytically active polymersome nanoreactors by flow cytometry
title_sort sorting catalytically active polymersome nanoreactors by flow cytometry
publishDate 2011
url https://hdl.handle.net/10356/93845
http://hdl.handle.net/10220/7282
_version_ 1681056937966305280
description Flow cytometry is a powerful technique for high-throughput, fluorescence-activated screening and sorting of cells (FACS). This methodology has been extended by Griffiths to the screening of water-in-oil microdroplets filled with an in vitro protein expression system.[1–6] The catalytic gene product was detected by the transformation of a co-encapsulated profluorescent substrate into a fluorescent product. Here we report a strategy that involves a versatile one-step preparation procedure of enzyme filled porous and stable polymeric capsules (polymersomes). Since the pores of the capsules are small enough to keep enzymes in, whereas these are sufficiently large to allow (profluorescent) substrates to enter, enzyme activity screening can be performed by the build-up of fluorescence, followed by FACS. To prevent the substrate from diffusing out of the capsules, a trapping agent was added inside the capsule. With this technology we were able to separate enzymatically active polymersomes from non-filled or non-active polymersomes.