Double spiral detection channel for on-chip chemiluminescence detection

• Main Authors: Lok, Khoi Seng, Kwok, Yien Chian, Nguyen, Nam-Trung
• Other Authors: School of Mechanical and Aerospace Engineering
• Format: Article
• Language: English
• Published: 2012
• Subjects: DRNTU::Engineering::Mechanical engineering
• Online Access: https://hdl.handle.net/10356/94018; http://hdl.handle.net/10220/8356

In this paper, we introduced a three-layered microchip that consists of a double spiral channel design for chemiluminescence (CL) detection and a passive micromixer to facilitate the mixing of reagents. The design with two overlapping spiral channels doubled the CL intensity emitted from the reaction as compared to the design with a single spiral channel. The addition of the passive micromixer improved the signal by 1.5 times. Luminol-based chemiluminescence reaction was used for the characterization of the device. This microchip was successfully tested with the determination of L-cysteine and uric acid.