A hydrogel-based enzyme-loaded polymersome reactor

In this study we report the immobilization of enzyme-containing polymersomes into a macromolecular hydrogel. Whereas free enzyme shows progressive leakage from the hydrogel in a period of days, leakage of the polymersome-protected enzyme is virtually absent. The preparation of the hydrogel occurs un...

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Main Authors: De Hoog, Hans-Peter M., Arends, Isabel W. C. E., Rowan, Alan E., Cornelissen, Jeroen J. L. M., Nolte, Roeland J. M.
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2011
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Online Access:https://hdl.handle.net/10356/94036
http://hdl.handle.net/10220/6970
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-940362023-07-14T15:53:16Z A hydrogel-based enzyme-loaded polymersome reactor De Hoog, Hans-Peter M. Arends, Isabel W. C. E. Rowan, Alan E. Cornelissen, Jeroen J. L. M. Nolte, Roeland J. M. School of Materials Science & Engineering DRNTU::Engineering::Materials::Biomaterials In this study we report the immobilization of enzyme-containing polymersomes into a macromolecular hydrogel. Whereas free enzyme shows progressive leakage from the hydrogel in a period of days, leakage of the polymersome-protected enzyme is virtually absent. The preparation of the hydrogel occurs under mild conditions and does not inhibit the activity of the encapsulated enzymes nor does it affect the structure of the polymersomes. The stability of the polymersome hydrogel architecture is demonstrated by the facile recycling of the polymersomes and their use in repeated reaction cycles. A ‘continuous-flow polymersome reactor’ is constructed in which substrate is added to the top of the reactor and product is collected at the bottom. This set-up allows the use of different enzymes and the processing of multiple substrates, as is demonstrated by the conversion of 2-methoxyphenyl acetate to tetraguaiacol in a reactor loaded with polymersome hydrogels containing the enzymes Candida antarcticalipase B (CALB) and glucose oxidase (GOx). Accepted version 2011-09-05T03:31:38Z 2019-12-06T18:49:38Z 2011-09-05T03:31:38Z 2019-12-06T18:49:38Z 2010 2010 Journal Article De Hoog, H. P. M., Arends, I. W. C. E., Rowan, A. E., Cornelissen, J. J. L. M., & Nolte, R. J. M. (2010). A hydrogel-based enzyme-loaded polymersome reactor. Nanoscale, 2, 709-716. 2040-3364 https://hdl.handle.net/10356/94036 http://hdl.handle.net/10220/6970 10.1039/b9nr00325h 155296 en Nanoscale © 2010 The Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by Nanoscale, The Royal Society of Chemistry.  It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document.  The published version is available at: [DOI: http://dx.doi.org/10.1039/b9nr00325h]. 8 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Materials::Biomaterials
spellingShingle DRNTU::Engineering::Materials::Biomaterials
De Hoog, Hans-Peter M.
Arends, Isabel W. C. E.
Rowan, Alan E.
Cornelissen, Jeroen J. L. M.
Nolte, Roeland J. M.
A hydrogel-based enzyme-loaded polymersome reactor
description In this study we report the immobilization of enzyme-containing polymersomes into a macromolecular hydrogel. Whereas free enzyme shows progressive leakage from the hydrogel in a period of days, leakage of the polymersome-protected enzyme is virtually absent. The preparation of the hydrogel occurs under mild conditions and does not inhibit the activity of the encapsulated enzymes nor does it affect the structure of the polymersomes. The stability of the polymersome hydrogel architecture is demonstrated by the facile recycling of the polymersomes and their use in repeated reaction cycles. A ‘continuous-flow polymersome reactor’ is constructed in which substrate is added to the top of the reactor and product is collected at the bottom. This set-up allows the use of different enzymes and the processing of multiple substrates, as is demonstrated by the conversion of 2-methoxyphenyl acetate to tetraguaiacol in a reactor loaded with polymersome hydrogels containing the enzymes Candida antarcticalipase B (CALB) and glucose oxidase (GOx).
author2 School of Materials Science & Engineering
author_facet School of Materials Science & Engineering
De Hoog, Hans-Peter M.
Arends, Isabel W. C. E.
Rowan, Alan E.
Cornelissen, Jeroen J. L. M.
Nolte, Roeland J. M.
format Article
author De Hoog, Hans-Peter M.
Arends, Isabel W. C. E.
Rowan, Alan E.
Cornelissen, Jeroen J. L. M.
Nolte, Roeland J. M.
author_sort De Hoog, Hans-Peter M.
title A hydrogel-based enzyme-loaded polymersome reactor
title_short A hydrogel-based enzyme-loaded polymersome reactor
title_full A hydrogel-based enzyme-loaded polymersome reactor
title_fullStr A hydrogel-based enzyme-loaded polymersome reactor
title_full_unstemmed A hydrogel-based enzyme-loaded polymersome reactor
title_sort hydrogel-based enzyme-loaded polymersome reactor
publishDate 2011
url https://hdl.handle.net/10356/94036
http://hdl.handle.net/10220/6970
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