Ferrofluid plug as valve and actuator for whole-cell PCR on chip

Ferrofluid plug was first used as valve and actuator to circumvent the evaporation of aqueous fluid in LOC. Moreover, this dynamic plug catered for aqueous fluid expansion during its heating in the microchannel. Whole-cell polymerase chain reaction (PCR) protocol was successfully implemented in a ma...

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Main Authors: Lok, Khoi Seng, Kwok, Yien Chian, Lee, Peter Peng Foo, Nguyen, Nam-Trung
Other Authors: School of Mechanical and Aerospace Engineering
Format: Article
Language:English
Published: 2012
Subjects:
Online Access:https://hdl.handle.net/10356/94209
http://hdl.handle.net/10220/8284
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-942092023-03-04T17:18:14Z Ferrofluid plug as valve and actuator for whole-cell PCR on chip Lok, Khoi Seng Kwok, Yien Chian Lee, Peter Peng Foo Nguyen, Nam-Trung School of Mechanical and Aerospace Engineering DRNTU::Engineering::Mechanical engineering Ferrofluid plug was first used as valve and actuator to circumvent the evaporation of aqueous fluid in LOC. Moreover, this dynamic plug catered for aqueous fluid expansion during its heating in the microchannel. Whole-cell polymerase chain reaction (PCR) protocol was successfully implemented in a magnetically actuated closed-loop PCR microchip system. Here, the ferrofluid plug was used to reduce the evaporation of the PCR sample in the thermal lysis step. A whole-cell PCR model was used to demonstrate this concept. The DNA plasmid, pUC19, was transformed into DH5α competent cells. Primer pairs were designed to amplify region of interest in pUC19. The transformants were cultured and selected. Whole-cell PCR was performed on intact bacteria to screen for the presence of pUC19, containing the region of interests. Serial dilutions demonstrated the limit of detection for this system to be four bacterial cells. PCR amplifications were verified with regular thermocyclers and results were comparable. Accepted version 2012-07-04T07:45:08Z 2019-12-06T18:52:29Z 2012-07-04T07:45:08Z 2019-12-06T18:52:29Z 2012 2012 Journal Article Lok, K. S., Kwok, Y. C., Lee, P. P. F., & Nguyen, N. T. (2012). Ferrofluid plug as valve and actuator for whole-cell PCR on chip. Sensors and Actuators B: Chemical, 166-167, (893–897). https://hdl.handle.net/10356/94209 http://hdl.handle.net/10220/8284 10.1016/j.snb.2012.03.001 164005 en Sensors and actuators B: chemical © 2012 Elsevier. This is the author created version of a work that has been peer reviewed and accepted for publication by Sensors and Actuators B: Chemical, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [DOI: http://dx.doi.org/10.1016/j.snb.2012.03.001] 21 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Mechanical engineering
spellingShingle DRNTU::Engineering::Mechanical engineering
Lok, Khoi Seng
Kwok, Yien Chian
Lee, Peter Peng Foo
Nguyen, Nam-Trung
Ferrofluid plug as valve and actuator for whole-cell PCR on chip
description Ferrofluid plug was first used as valve and actuator to circumvent the evaporation of aqueous fluid in LOC. Moreover, this dynamic plug catered for aqueous fluid expansion during its heating in the microchannel. Whole-cell polymerase chain reaction (PCR) protocol was successfully implemented in a magnetically actuated closed-loop PCR microchip system. Here, the ferrofluid plug was used to reduce the evaporation of the PCR sample in the thermal lysis step. A whole-cell PCR model was used to demonstrate this concept. The DNA plasmid, pUC19, was transformed into DH5α competent cells. Primer pairs were designed to amplify region of interest in pUC19. The transformants were cultured and selected. Whole-cell PCR was performed on intact bacteria to screen for the presence of pUC19, containing the region of interests. Serial dilutions demonstrated the limit of detection for this system to be four bacterial cells. PCR amplifications were verified with regular thermocyclers and results were comparable.
author2 School of Mechanical and Aerospace Engineering
author_facet School of Mechanical and Aerospace Engineering
Lok, Khoi Seng
Kwok, Yien Chian
Lee, Peter Peng Foo
Nguyen, Nam-Trung
format Article
author Lok, Khoi Seng
Kwok, Yien Chian
Lee, Peter Peng Foo
Nguyen, Nam-Trung
author_sort Lok, Khoi Seng
title Ferrofluid plug as valve and actuator for whole-cell PCR on chip
title_short Ferrofluid plug as valve and actuator for whole-cell PCR on chip
title_full Ferrofluid plug as valve and actuator for whole-cell PCR on chip
title_fullStr Ferrofluid plug as valve and actuator for whole-cell PCR on chip
title_full_unstemmed Ferrofluid plug as valve and actuator for whole-cell PCR on chip
title_sort ferrofluid plug as valve and actuator for whole-cell pcr on chip
publishDate 2012
url https://hdl.handle.net/10356/94209
http://hdl.handle.net/10220/8284
_version_ 1759856256018808832