An on-nanoparticle rolling-circle amplification platform for ultrasensitive protein detection in biological fluids

An on-nanoparticle rolling-circle amplification (nanoRCA) strategy allows the visual detection of 30 molecules of carcinoembryonic antigen with at least 1010-fold selectivity over a range of noncognate proteins. The nanoRCA assay is applicable to the detection of two tumor markers in biological flui...

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Bibliographic Details
Main Authors: Yan, Juan, Song, Shiping, Li, Bing, Zhang, Qingzhi, Huang, Qing, Zhang, Hua, Fan, Chunhai
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2012
Subjects:
Online Access:https://hdl.handle.net/10356/94271
http://hdl.handle.net/10220/8578
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Institution: Nanyang Technological University
Language: English
Description
Summary:An on-nanoparticle rolling-circle amplification (nanoRCA) strategy allows the visual detection of 30 molecules of carcinoembryonic antigen with at least 1010-fold selectivity over a range of noncognate proteins. The nanoRCA assay is applicable to the detection of two tumor markers in biological fluids: prostate-specific antigen in spiked sera and interleukin-8 in saliva.