An on-nanoparticle rolling-circle amplification platform for ultrasensitive protein detection in biological fluids

• Main Authors: Yan, Juan, Song, Shiping, Li, Bing, Zhang, Qingzhi, Huang, Qing, Zhang, Hua, Fan, Chunhai
• Other Authors: School of Materials Science & Engineering
• Format: Article
• Language: English
• Published: 2012
• Subjects: DRNTU::Engineering::Materials
• Online Access: https://hdl.handle.net/10356/94271; http://hdl.handle.net/10220/8578
• Institution: Nanyang Technological University

An on-nanoparticle rolling-circle amplification (nanoRCA) strategy allows the visual detection of 30 molecules of carcinoembryonic antigen with at least 1010-fold selectivity over a range of noncognate proteins. The nanoRCA assay is applicable to the detection of two tumor markers in biological fluids: prostate-specific antigen in spiked sera and interleukin-8 in saliva.