Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy
Exocytosis of neurotransmitter or hormone-filled vesicles is a highly dynamic process regulated by various proteins and lipids. As mainly revealed indirectly by the electrophysiological methods, exocytosis is believed to involve multiple kinetic steps in which vesicles tr...
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| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/94319 http://hdl.handle.net/10220/7522 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | Exocytosis of neurotransmitter or hormone-filled
vesicles is a highly dynamic process regulated by various
proteins and lipids. As mainly revealed indirectly by the
electrophysiological methods, exocytosis is believed to
involve multiple kinetic steps in which vesicles transit from
one state to another. Using total internal reflection
fluorescence microscopy which enables direct visualization
of individual vesicles, we developed an analytical framework
to track and analyze vesicle dynamics. We demonstrated
that all subplasmalemmal vesicles generally undergo
constant and caged Brownian motion. And they can be
classified into three populations that differ in their motion
characteristics and fusion competence. Furthermore, we
showed that these vesicle pools are differentially modulated
by phorbol-12-myristate-13-acetate, a phorbol ester analog
to endogenous diacylglycerol, through both protein-kinase-
C-dependent and -independent pathways. |
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