Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly

During mitosis, the nuclear envelope merges with the endoplasmic reticulum (ER), and nuclear pore complexes are disassembled. In a current model for reassembly after mitosis, the nuclear envelope forms by a reshaping of ER tubules. For the assembly of pores, two major models have been proposed. In t...

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Main Authors: Lu, Lei, Ladinsky, Mark S., Kirchhausen, Tomas.
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2011
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Online Access:https://hdl.handle.net/10356/94406
http://hdl.handle.net/10220/7161
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-944062023-02-28T17:03:55Z Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly Lu, Lei Ladinsky, Mark S. Kirchhausen, Tomas. School of Biological Sciences DRNTU::Science::Biological sciences::Molecular biology During mitosis, the nuclear envelope merges with the endoplasmic reticulum (ER), and nuclear pore complexes are disassembled. In a current model for reassembly after mitosis, the nuclear envelope forms by a reshaping of ER tubules. For the assembly of pores, two major models have been proposed. In the insertion model, nuclear pore complexes are embedded in the nuclear envelope after their formation. In the prepore model, nucleoporins assemble on the chromatin as an intermediate nuclear pore complex before nuclear envelope formation. Using live-cell imaging and electron microscope tomography, we find that the mitotic assembly of the nuclear envelope primarily originates from ER cisternae. Moreover, the nuclear pore complexes assemble only on the already formed nuclear envelope. Indeed, all the chromatin-associated Nup107–160 complexes are in single units instead of assembled prepores. We therefore propose that the postmitotic nuclear envelope assembles directly from ER cisternae followed by membrane-dependent insertion of nuclear pore complexes. Published version 2011-10-05T06:58:11Z 2019-12-06T18:55:31Z 2011-10-05T06:58:11Z 2019-12-06T18:55:31Z 2011 2011 Journal Article Lu, L., Ladinsky, M. S., & Kirchhausen, T. (2011). Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly. The Journal of Cell Biology, 194(3), 425-440. https://hdl.handle.net/10356/94406 http://hdl.handle.net/10220/7161 10.1083/jcb.201012063 21825076 en The journal of cell Biology © 2011 Lu et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Molecular biology
spellingShingle DRNTU::Science::Biological sciences::Molecular biology
Lu, Lei
Ladinsky, Mark S.
Kirchhausen, Tomas.
Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly
description During mitosis, the nuclear envelope merges with the endoplasmic reticulum (ER), and nuclear pore complexes are disassembled. In a current model for reassembly after mitosis, the nuclear envelope forms by a reshaping of ER tubules. For the assembly of pores, two major models have been proposed. In the insertion model, nuclear pore complexes are embedded in the nuclear envelope after their formation. In the prepore model, nucleoporins assemble on the chromatin as an intermediate nuclear pore complex before nuclear envelope formation. Using live-cell imaging and electron microscope tomography, we find that the mitotic assembly of the nuclear envelope primarily originates from ER cisternae. Moreover, the nuclear pore complexes assemble only on the already formed nuclear envelope. Indeed, all the chromatin-associated Nup107–160 complexes are in single units instead of assembled prepores. We therefore propose that the postmitotic nuclear envelope assembles directly from ER cisternae followed by membrane-dependent insertion of nuclear pore complexes.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Lu, Lei
Ladinsky, Mark S.
Kirchhausen, Tomas.
format Article
author Lu, Lei
Ladinsky, Mark S.
Kirchhausen, Tomas.
author_sort Lu, Lei
title Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly
title_short Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly
title_full Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly
title_fullStr Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly
title_full_unstemmed Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly
title_sort formation of the postmitotic nuclear envelope from extended er cisternae precedes nuclear pore assembly
publishDate 2011
url https://hdl.handle.net/10356/94406
http://hdl.handle.net/10220/7161
_version_ 1759858399756943360