Design of β-hairpin peptides for modulation of cell adhesion by β-turn constraint
The CD2−CD58 interaction in immune regulation and disease pathology has provided new targets for developing potential immunosuppressive agents. In the present study, we report the introduction of constraints to generate β-hairpin structures from the strand sequences of CD2 protein. The β-hairpin str...
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sg-ntu-dr.10356-945272020-03-07T12:18:17Z Design of β-hairpin peptides for modulation of cell adhesion by β-turn constraint Satyanarayanajois, Seetharama D. Giddu, Sumana Subramanian, Vivekanandan Yoon, Ho Sup School of Biological Sciences DRNTU::Science::Biological sciences::Molecular biology The CD2−CD58 interaction in immune regulation and disease pathology has provided new targets for developing potential immunosuppressive agents. In the present study, we report the introduction of constraints to generate β-hairpin structures from the strand sequences of CD2 protein. The β-hairpin structures were induced in the designed peptides by introducing Pro-Gly sequences in the peptides. Results from NMR and MD simulation indicated that the peptides exhibited β-turn structure at the X-Pro-Gly-Y sequence and formed the β-hairpin structure in solution. The ability of these peptides to inhibit cell adhesion was evaluated by two cell adhesion assays. Among the peptides studied (1−4) (P1−P4), peptides 2−4 were able to inhibit cell adhesion between Jurkat cells and SRBC nearly 50% at 180 μM, and 80% inhibition between Jurkat cells and Caco-2 cells was seen at 90 μM. Peptide 1 did not show significant inhibition activity compared to control. 2012-01-26T01:34:47Z 2019-12-06T18:57:27Z 2012-01-26T01:34:47Z 2019-12-06T18:57:27Z 2009 2009 Journal Article Giddu, S., Subramanian, V., Yoon, H. S., & Satyanarayanajois, S. D. (2009). Design of β-Hairpin Peptides for Modulation of Cell Adhesion by β-Turn Constraint. Journal of Medicinal Chemistry, 52(3), 726-736. https://hdl.handle.net/10356/94527 http://hdl.handle.net/10220/7477 10.1021/jm8008212 en Journal of medicinal Chemistry © 2009 American Chemical Society. |
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DRNTU::Science::Biological sciences::Molecular biology Satyanarayanajois, Seetharama D. Giddu, Sumana Subramanian, Vivekanandan Yoon, Ho Sup Design of β-hairpin peptides for modulation of cell adhesion by β-turn constraint |
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The CD2−CD58 interaction in immune regulation and disease pathology has provided new targets for developing potential immunosuppressive agents. In the present study, we report the introduction of constraints to generate β-hairpin structures from the strand sequences of CD2 protein. The β-hairpin structures were induced in the designed peptides by introducing Pro-Gly sequences in the peptides. Results from NMR and MD simulation indicated that the peptides exhibited β-turn structure at the X-Pro-Gly-Y sequence and formed the β-hairpin structure in solution. The ability of these peptides to inhibit cell adhesion was evaluated by two cell adhesion assays. Among the peptides studied (1−4) (P1−P4), peptides 2−4 were able to inhibit cell adhesion between Jurkat cells and SRBC nearly 50% at 180 μM, and 80% inhibition between Jurkat cells and Caco-2 cells was seen at 90 μM. Peptide 1 did not show significant inhibition activity compared to control. |
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School of Biological Sciences |
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School of Biological Sciences Satyanarayanajois, Seetharama D. Giddu, Sumana Subramanian, Vivekanandan Yoon, Ho Sup |
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Article |
author |
Satyanarayanajois, Seetharama D. Giddu, Sumana Subramanian, Vivekanandan Yoon, Ho Sup |
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Satyanarayanajois, Seetharama D. |
title |
Design of β-hairpin peptides for modulation of cell adhesion by β-turn constraint |
title_short |
Design of β-hairpin peptides for modulation of cell adhesion by β-turn constraint |
title_full |
Design of β-hairpin peptides for modulation of cell adhesion by β-turn constraint |
title_fullStr |
Design of β-hairpin peptides for modulation of cell adhesion by β-turn constraint |
title_full_unstemmed |
Design of β-hairpin peptides for modulation of cell adhesion by β-turn constraint |
title_sort |
design of β-hairpin peptides for modulation of cell adhesion by β-turn constraint |
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2012 |
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https://hdl.handle.net/10356/94527 http://hdl.handle.net/10220/7477 |
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