Enhanced electrophoretic DNA separation in photonic crystal fiber
Joule heating generated by the electrical current in capillary electrophoresis leads to a temperature gradient along the separation channel and consequently affects the separation quality. We describe a method of reducing the Joule heating effect by incorporating photonic crystal fiber into a micro...
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sg-ntu-dr.10356-946592023-03-04T17:15:25Z Enhanced electrophoretic DNA separation in photonic crystal fiber Sun, Yi Nguyen, Nam-Trung Kwok, Yien Chian School of Mechanical and Aerospace Engineering DRNTU::Engineering::Mechanical engineering Joule heating generated by the electrical current in capillary electrophoresis leads to a temperature gradient along the separation channel and consequently affects the separation quality. We describe a method of reducing the Joule heating effect by incorporating photonic crystal fiber into a micro capillary electrophoresis chip. The photonic crystal fiber consists of a bundle of extremely narrow hollow channels, which ideally work as separation columns. Electrophoretic separation of DNA fragments was simultaneously but independently carried out in 54 narrow capillaries with a diameter of 3.7 μm each. The capillary bundle offers more efficient heat dissipation owing to the high surface-to-volume ratio. Under the same electrical field strength, notable improvement in resolution was obtained in the capillary bundle chip. Accepted version 2012-04-12T05:58:47Z 2019-12-06T18:59:48Z 2012-04-12T05:58:47Z 2019-12-06T18:59:48Z 2009 2009 Journal Article Sun, Y., Nguyen, N. T., & Kwok, Y. C. (2009). Enhanced electrophoretic DNA separation in photonic crystal fiber. Analytical and Bioanalytical Chemistry, 394(6), 1707-1710. https://hdl.handle.net/10356/94659 http://hdl.handle.net/10220/7752 10.1007/s00216-009-2833-6 152444 en Analytical and bioanalytical chemistry © 2009 Springer-Verlag. This is the author created version of a work that has been peer reviewed and accepted for publication by Analytical and bioanalytical chemistry, Springer-Verlag. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: http://dx.doi.org/10.1007/s00216-009-2833-6. application/pdf |
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DRNTU::Engineering::Mechanical engineering Sun, Yi Nguyen, Nam-Trung Kwok, Yien Chian Enhanced electrophoretic DNA separation in photonic crystal fiber |
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Joule heating generated by the electrical current in capillary electrophoresis leads to a temperature gradient along the separation channel and consequently affects the separation quality. We describe a method of reducing the Joule heating effect by incorporating photonic crystal fiber into a micro capillary electrophoresis chip. The photonic crystal fiber consists of a bundle of extremely narrow hollow channels, which ideally work as separation columns. Electrophoretic separation of DNA fragments was simultaneously but independently carried out in 54 narrow capillaries with a diameter of 3.7 μm each. The capillary bundle offers more efficient heat dissipation owing to the high surface-to-volume ratio. Under the same electrical field strength, notable improvement in resolution was obtained in the capillary bundle chip. |
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School of Mechanical and Aerospace Engineering |
author_facet |
School of Mechanical and Aerospace Engineering Sun, Yi Nguyen, Nam-Trung Kwok, Yien Chian |
format |
Article |
author |
Sun, Yi Nguyen, Nam-Trung Kwok, Yien Chian |
author_sort |
Sun, Yi |
title |
Enhanced electrophoretic DNA separation in photonic crystal fiber |
title_short |
Enhanced electrophoretic DNA separation in photonic crystal fiber |
title_full |
Enhanced electrophoretic DNA separation in photonic crystal fiber |
title_fullStr |
Enhanced electrophoretic DNA separation in photonic crystal fiber |
title_full_unstemmed |
Enhanced electrophoretic DNA separation in photonic crystal fiber |
title_sort |
enhanced electrophoretic dna separation in photonic crystal fiber |
publishDate |
2012 |
url |
https://hdl.handle.net/10356/94659 http://hdl.handle.net/10220/7752 |
_version_ |
1759853818926858240 |