Crystallization and preliminary X-ray crystallographic analysis of subunit F (F1-94), an essential coupling subunit of the eukaryotic V1VO-ATPase from Saccharomyces cerevisiae

• Main Authors: Basak, Sandip, Balakrishna, Asha Manikkoth, Manimekalai, Malathy Sony Subramanian, Grüber, Gerhard
• 其他作者: School of Biological Sciences
• 格式: Article
• 語言: English
• 出版: 2013
• 主題: DRNTU::Science::Chemistry::Biochemistry; DRNTU::Science::Chemistry::Crystallography
• 在線閱讀: https://hdl.handle.net/10356/95360; http://hdl.handle.net/10220/9180
• 機構: Nanyang Technological University

V-ATPases are very complex multi-subunit enzymes which function as proton-pumping rotary nanomotors. The rotary and coupling subunit F (F1-94) was crystallized by the hanging-drop vapour-diffusion method. The native crystals diffracted to a resolution of 2.64 Å and belonged to space group C2221, wit...