Visual cocaine detection with gold nanoparticles and rationally engineered aptamer structures
A novel bioassay strategy is designed to detect small-molecule targets such as cocaine, potassium, and adenosine, based on gold nanoparticles (AuNPs) and engineered DNA aptamers. In this design, an aptamer is engineered to be two pieces of random, coil-like single-stranded DNA, which reassembles int...
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sg-ntu-dr.10356-955892020-06-01T10:26:48Z Visual cocaine detection with gold nanoparticles and rationally engineered aptamer structures Zhang, Juan Wang, Lihua Pan, Dun Song, Shiping Boey, Freddy Yin Chiang Zhang, Hua Fan, Chunhai School of Materials Science & Engineering DRNTU::Engineering::Materials A novel bioassay strategy is designed to detect small-molecule targets such as cocaine, potassium, and adenosine, based on gold nanoparticles (AuNPs) and engineered DNA aptamers. In this design, an aptamer is engineered to be two pieces of random, coil-like single-stranded DNA, which reassembles into the intact aptamer tertiary structure in the presence of the specific target. AuNPs can effectively differentiate between these two states via their characteristic surface-plasmon resonance-based color change. Using this method, cocaine in the low-micromolar range is selectively detected within minutes. This strategy is also shown to be generic and applicable to the detection of several other small-molecule targets. 2012-09-24T01:00:02Z 2019-12-06T19:17:56Z 2012-09-24T01:00:02Z 2019-12-06T19:17:56Z 2008 2008 Journal Article Zhang, J., Wang, L., Pan, D., Song, S., Boey, F. Y. C., Zhang, H., et al. (2008). Visual cocaine detection with gold nanoparticles and rationally engineered aptamer structures. Small, 4(8), 1196-1200. 1613-6829 https://hdl.handle.net/10356/95589 http://hdl.handle.net/10220/8604 10.1002/smll.200800057 en Small © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. |
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DRNTU::Engineering::Materials Zhang, Juan Wang, Lihua Pan, Dun Song, Shiping Boey, Freddy Yin Chiang Zhang, Hua Fan, Chunhai Visual cocaine detection with gold nanoparticles and rationally engineered aptamer structures |
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A novel bioassay strategy is designed to detect small-molecule targets such as cocaine, potassium, and adenosine, based on gold nanoparticles (AuNPs) and engineered DNA aptamers. In this design, an aptamer is engineered to be two pieces of random, coil-like single-stranded DNA, which reassembles into the intact aptamer tertiary structure in the presence of the specific target. AuNPs can effectively differentiate between these two states via their characteristic surface-plasmon resonance-based color change. Using this method, cocaine in the low-micromolar range is selectively detected within minutes. This strategy is also shown to be generic and applicable to the detection of several other small-molecule targets. |
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School of Materials Science & Engineering |
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School of Materials Science & Engineering Zhang, Juan Wang, Lihua Pan, Dun Song, Shiping Boey, Freddy Yin Chiang Zhang, Hua Fan, Chunhai |
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Article |
author |
Zhang, Juan Wang, Lihua Pan, Dun Song, Shiping Boey, Freddy Yin Chiang Zhang, Hua Fan, Chunhai |
author_sort |
Zhang, Juan |
title |
Visual cocaine detection with gold nanoparticles and rationally engineered aptamer structures |
title_short |
Visual cocaine detection with gold nanoparticles and rationally engineered aptamer structures |
title_full |
Visual cocaine detection with gold nanoparticles and rationally engineered aptamer structures |
title_fullStr |
Visual cocaine detection with gold nanoparticles and rationally engineered aptamer structures |
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Visual cocaine detection with gold nanoparticles and rationally engineered aptamer structures |
title_sort |
visual cocaine detection with gold nanoparticles and rationally engineered aptamer structures |
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2012 |
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https://hdl.handle.net/10356/95589 http://hdl.handle.net/10220/8604 |
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