Pan-pathway based interaction profiling of FDA-approved nucleoside and nucleobase analogs with enzymes of the human nucleotide metabolism

• Main Authors: Egeblad, Louise, Welin, Martin, Flodin, Susanne, Gräslund, Susanne, Wang, Liya, Balzarini, Jan, Eriksson, Staffan, Nordlund, Pär
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2013
• Online Access: https://hdl.handle.net/10356/95769; http://hdl.handle.net/10220/9364
• Institution: Nanyang Technological University

To identify interactions a nucleoside analog library (NAL) consisting of 45 FDA-approved nucleoside analogs was screened against 23 enzymes of the human nucleotide metabolism using a thermal shift assay. The method was validated with deoxycytidine kinase; eight interactions known from the literature...