Enhancing mesenchymal stem cell response using uniaxially stretched poly(ε-caprolactone) film micropatterns for vascular tissue engineering application

Regeneration of tunica media with anisotropic architecture still remains a challenging issue for vascular tissue engineering (TE). Herein, we present the development of flexible poly(ε-caprolactone) (PCL) film micropatterns to regulate mesenchymal stem cells (MSCs) function for tunica media construc...

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Main Authors: Wang, Zu-yong, Teoh, Swee Hin, Johana, Nuryanti Binti, Khoon Chong, Mark Seow, Teo, Erin Yiling, Hong, Ming-hui, Yen Chan, Jerry Kok, San Thian, Eng
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2015
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Online Access:https://hdl.handle.net/10356/96200
http://hdl.handle.net/10220/38470
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-962002023-12-29T06:45:08Z Enhancing mesenchymal stem cell response using uniaxially stretched poly(ε-caprolactone) film micropatterns for vascular tissue engineering application Wang, Zu-yong Teoh, Swee Hin Johana, Nuryanti Binti Khoon Chong, Mark Seow Teo, Erin Yiling Hong, Ming-hui Yen Chan, Jerry Kok San Thian, Eng School of Chemical and Biomedical Engineering DRNTU::Science::Medicine::Tissue engineering Regeneration of tunica media with anisotropic architecture still remains a challenging issue for vascular tissue engineering (TE). Herein, we present the development of flexible poly(ε-caprolactone) (PCL) film micropatterns to regulate mesenchymal stem cells (MSCs) function for tunica media construction. Results showed that uniaxial thermal stretching of PCL films resulted in topographical micropatterns comprising of ridges/grooves, and improved mechanical properties, including yield stress, Young's modulus, and fracture stress without sacrificing film elasticity. Culturing on such PCL film micropatterns, MSCs self-aligned along the ridges with a more elongated morphology as compared to that of the un-stretched film group. Moreover, MSCs obtained a contractile SMCs-like phenotype, with ordered organization of cellular stress filaments and upregulated expression of the contractile makers, including SM-α-actin, calponin, and SM-MHC. The PCL film micropatterns could be rolled into a small-diameter 3D tubular scaffold with circumferential anisotropy of ridges/grooves, and in the incorporation of MSCs, which facilitated a hybrid sandwich-like vascular wall construction with ordered cell architecture similar to that of the tunica media. These results provide insights of how geometric cues are able to regulate stem cells with desired functions and have significant implications for the designing of a functionalized vascular TE scaffold with appropriate topographical geometries for guiding tunica media regeneration with microscale control of cell alignment and genetic expression. Published version 2015-08-19T08:29:45Z 2019-12-06T19:27:00Z 2015-08-19T08:29:45Z 2019-12-06T19:27:00Z 2014 2014 Journal Article Wang, Z. y., Teoh, S. H., Johana, N. B., Khoon Chong, M. S., Teo, E. Y., Hong, M. h., Yen Chan, J. K., & San Thian, E. (2014). Enhancing mesenchymal stem cell response using uniaxially stretched poly(ε-caprolactone) film micropatterns for vascular tissue engineering application. J. Mater. Chem. B, 2(35), 5898-5909. 2050-750X https://hdl.handle.net/10356/96200 http://hdl.handle.net/10220/38470 10.1039/C4TB00522H en Journal of materials chemistry B © 2014 The Royal Society of Chemistry. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. 12 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Medicine::Tissue engineering
spellingShingle DRNTU::Science::Medicine::Tissue engineering
Wang, Zu-yong
Teoh, Swee Hin
Johana, Nuryanti Binti
Khoon Chong, Mark Seow
Teo, Erin Yiling
Hong, Ming-hui
Yen Chan, Jerry Kok
San Thian, Eng
Enhancing mesenchymal stem cell response using uniaxially stretched poly(ε-caprolactone) film micropatterns for vascular tissue engineering application
description Regeneration of tunica media with anisotropic architecture still remains a challenging issue for vascular tissue engineering (TE). Herein, we present the development of flexible poly(ε-caprolactone) (PCL) film micropatterns to regulate mesenchymal stem cells (MSCs) function for tunica media construction. Results showed that uniaxial thermal stretching of PCL films resulted in topographical micropatterns comprising of ridges/grooves, and improved mechanical properties, including yield stress, Young's modulus, and fracture stress without sacrificing film elasticity. Culturing on such PCL film micropatterns, MSCs self-aligned along the ridges with a more elongated morphology as compared to that of the un-stretched film group. Moreover, MSCs obtained a contractile SMCs-like phenotype, with ordered organization of cellular stress filaments and upregulated expression of the contractile makers, including SM-α-actin, calponin, and SM-MHC. The PCL film micropatterns could be rolled into a small-diameter 3D tubular scaffold with circumferential anisotropy of ridges/grooves, and in the incorporation of MSCs, which facilitated a hybrid sandwich-like vascular wall construction with ordered cell architecture similar to that of the tunica media. These results provide insights of how geometric cues are able to regulate stem cells with desired functions and have significant implications for the designing of a functionalized vascular TE scaffold with appropriate topographical geometries for guiding tunica media regeneration with microscale control of cell alignment and genetic expression.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Wang, Zu-yong
Teoh, Swee Hin
Johana, Nuryanti Binti
Khoon Chong, Mark Seow
Teo, Erin Yiling
Hong, Ming-hui
Yen Chan, Jerry Kok
San Thian, Eng
format Article
author Wang, Zu-yong
Teoh, Swee Hin
Johana, Nuryanti Binti
Khoon Chong, Mark Seow
Teo, Erin Yiling
Hong, Ming-hui
Yen Chan, Jerry Kok
San Thian, Eng
author_sort Wang, Zu-yong
title Enhancing mesenchymal stem cell response using uniaxially stretched poly(ε-caprolactone) film micropatterns for vascular tissue engineering application
title_short Enhancing mesenchymal stem cell response using uniaxially stretched poly(ε-caprolactone) film micropatterns for vascular tissue engineering application
title_full Enhancing mesenchymal stem cell response using uniaxially stretched poly(ε-caprolactone) film micropatterns for vascular tissue engineering application
title_fullStr Enhancing mesenchymal stem cell response using uniaxially stretched poly(ε-caprolactone) film micropatterns for vascular tissue engineering application
title_full_unstemmed Enhancing mesenchymal stem cell response using uniaxially stretched poly(ε-caprolactone) film micropatterns for vascular tissue engineering application
title_sort enhancing mesenchymal stem cell response using uniaxially stretched poly(ε-caprolactone) film micropatterns for vascular tissue engineering application
publishDate 2015
url https://hdl.handle.net/10356/96200
http://hdl.handle.net/10220/38470
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