P53 gene targeting by homologous recombination in fish ES cells

• Main Authors: Yan, Yan, Hong, Ni, Chen, Tiansheng, Li, Mingyou, Wang, Tiansu, Guan, Guijun, Qiao, Yongkang, Chen, Songlin, Schartl, Manfred, Hong, Yunhan, Li, Chang Ming
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2013
• Subjects: DRNTU::Science::Biological sciences::Microbiology::Microorganisms
• Online Access: https://hdl.handle.net/10356/96391; http://hdl.handle.net/10220/9878
• Institution: Nanyang Technological University

Gene targeting (GT) provides a powerful tool for the generation of precise genetic alterations in embryonic stem (ES) cells to elucidate gene function and create animal models for human diseases. This technology has, however, been limited to mouse and rat. We have previously established ES cell li...