Improving ethanol tolerance of Escherichia coli by rewiring Its global regulator cAMP receptor protein (CRP)

• Main Authors: Chong, Huiqing, Huang, Lei, Yeow, Jianwei, Wang, Ivy, Zhang, Hongfang, Song, Hao, Jiang, Rongrong
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2013
• Subjects: DRNTU::Science::Biological sciences::Genetics
• Online Access: https://hdl.handle.net/10356/96553; http://hdl.handle.net/10220/9887
• Institution: Nanyang Technological University

A major challenge in bioethanol fermentation is the low tolerance of the microbial host towards the end product bioethanol. Here we report to improve the ethanol tolerance of E. coli from the transcriptional level by engineering its global transcription factor cAMP receptor protein (CRP), which is...