High productivity chromatography refolding process for Hepatitis B Virus X (HBx) protein guided by statistical design of experiment studies

• Main Authors: Basu, Anindya, Leong, Susanna Su Jan
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2013
• Subjects: DRNTU::Engineering::Chemical engineering
• Online Access: https://hdl.handle.net/10356/96977; http://hdl.handle.net/10220/11668
• Institution: Nanyang Technological University

The Hepatitis B Virus X (HBx) protein is a potential therapeutic target for the treatment of hepatocellular carcinoma. However, consistent expression of the protein as insoluble inclusion bodies in bacteria host systems has largely hindered HBx manufacturing via economical biosynthesis routes, there...