Lasioglossin-III : antimicrobial characterization and feasibility study for immobilization applications
Prevalent bacterial colonization and subsequent biofilm formation in biomedical implants demand for improved antimicrobial properties of these devices. To address this problem, immobilizing antimicrobial peptides (AMPs) on implants is a promising solution because of their biocompatibility and lesser...
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sg-ntu-dr.10356-974112023-07-14T15:54:22Z Lasioglossin-III : antimicrobial characterization and feasibility study for immobilization applications Mishra, Biswajit Basu, Anindya Saravanan, Rathi Xiang, Li Yang, Lim Kai Leong, Susanna Su Jan School of Chemical and Biomedical Engineering DRNTU::Science::Biological sciences::Microbiology::Bacteria Prevalent bacterial colonization and subsequent biofilm formation in biomedical implants demand for improved antimicrobial properties of these devices. To address this problem, immobilizing antimicrobial peptides (AMPs) on implants is a promising solution because of their biocompatibility and lesser likelihood to incur pathogen resistance. This study presents a systematic approach towards evaluating the feasibility of Lasioglossin-III (Lasio-III) (a new bee venom AMP, found in Lasioglossum laticeps) to be tethered onto biodevices. Antimicrobial characterization of Lasio-III in solution confirms the peptide’s membranolytic mode of action and its salt-resistant, broad antimicrobial spectrum activity and anti-biofilm properties against Gram negative and Gram positive bacteria. Lassio-III was covalently immobilized on silicon surfaces using APTES and PEG spacers of varying lengths. Surface characterization of the AMP-immobilized silicon was done using water contact angle measurements, XPS analysis and ellipsometry. Even at modest surface peptide concentrations of y180 ng cm22 , Lassio-III showed antibacterial activities which were further enhanced with increasing PEG spacer lengths, as determined by live CFU counting and ATP leakage experiments. This proof-of-concept study demonstrates the potential of Lasio-III as an antimicrobial coating candidate. Accepted version 2013-06-04T08:19:32Z 2019-12-06T19:42:23Z 2013-06-04T08:19:32Z 2019-12-06T19:42:23Z 2013 2013 Journal Article Mishra, B., Basu, A., Saravanan, R., Xiang, L., Yang, L. K., & Leong, S. S. J. (2013). Lasioglossin-III: antimicrobial characterization and feasibility study for immobilization applications. RSC Advances, 3(24), 9534-9543. https://hdl.handle.net/10356/97411 http://hdl.handle.net/10220/10057 10.1039/C3RA40887F 171651 en RSC advances © 2013 Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by RSC advances, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [DOI:http://dx.doi.org/10.1039/C3RA40887F]. application/pdf |
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DRNTU::Science::Biological sciences::Microbiology::Bacteria Mishra, Biswajit Basu, Anindya Saravanan, Rathi Xiang, Li Yang, Lim Kai Leong, Susanna Su Jan Lasioglossin-III : antimicrobial characterization and feasibility study for immobilization applications |
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Prevalent bacterial colonization and subsequent biofilm formation in biomedical implants demand for improved antimicrobial properties of these devices. To address this problem, immobilizing antimicrobial peptides (AMPs) on implants is a promising solution because of their biocompatibility and lesser likelihood to incur pathogen resistance. This study presents a systematic approach towards evaluating the feasibility of Lasioglossin-III (Lasio-III) (a new bee venom AMP, found in Lasioglossum laticeps) to be tethered onto biodevices. Antimicrobial characterization of Lasio-III in solution confirms the peptide’s membranolytic
mode of action and its salt-resistant, broad antimicrobial spectrum activity and anti-biofilm properties against Gram negative and Gram positive bacteria. Lassio-III was covalently immobilized on silicon surfaces using APTES and PEG spacers of varying lengths. Surface characterization of the AMP-immobilized silicon was done using water contact angle measurements, XPS analysis and ellipsometry. Even at modest surface peptide concentrations of y180 ng cm22 , Lassio-III showed antibacterial activities which were further
enhanced with increasing PEG spacer lengths, as determined by live CFU counting and ATP leakage experiments. This proof-of-concept study demonstrates the potential of Lasio-III as an antimicrobial coating candidate. |
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School of Chemical and Biomedical Engineering |
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School of Chemical and Biomedical Engineering Mishra, Biswajit Basu, Anindya Saravanan, Rathi Xiang, Li Yang, Lim Kai Leong, Susanna Su Jan |
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Article |
author |
Mishra, Biswajit Basu, Anindya Saravanan, Rathi Xiang, Li Yang, Lim Kai Leong, Susanna Su Jan |
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Mishra, Biswajit |
title |
Lasioglossin-III : antimicrobial characterization and feasibility study for immobilization applications |
title_short |
Lasioglossin-III : antimicrobial characterization and feasibility study for immobilization applications |
title_full |
Lasioglossin-III : antimicrobial characterization and feasibility study for immobilization applications |
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Lasioglossin-III : antimicrobial characterization and feasibility study for immobilization applications |
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Lasioglossin-III : antimicrobial characterization and feasibility study for immobilization applications |
title_sort |
lasioglossin-iii : antimicrobial characterization and feasibility study for immobilization applications |
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2013 |
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https://hdl.handle.net/10356/97411 http://hdl.handle.net/10220/10057 |
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