Engineering global transcription factor cyclic AMP receptor protein of Escherichia coli for improved 1-butanol tolerance

• Main Authors: Zhang, Hongfang, Chong, Huiqing, Ching, Chi Bun, Song, Hao, Jiang, Rongrong
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2013
• Subjects: DRNTU::Engineering::Chemical engineering::Biotechnology
• Online Access: https://hdl.handle.net/10356/98865; http://hdl.handle.net/10220/12518
• Institution: Nanyang Technological University

One major challenge in biofuel production, including biobutanol production, is the low tolerance of the microbial host towards increasing biofuel concentration during fermentation. Here, we have demonstrated that Escherichia coli 1-butanol tolerance can be greatly enhanced through random mutagenesis...