Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution

The design of a novel multicolor fluorescent gold nanoprobe for homogeneous detection of small-molecule targets is reported, which combines the specific binding abilities of aptamers with the ultrahigh quenching ability of gold nanoparticles (AuNPs). Dye-tagged aptamers and their complementary seque...

Full description

Saved in:
Bibliographic Details
Main Authors: Fan, Chunhai, Zhang, Juan, Wang, Lihua, Zhang, Hua, Boey, Freddy Yin Chiang, Song, Shiping
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2012
Subjects:
Online Access:https://hdl.handle.net/10356/99641
http://hdl.handle.net/10220/8584
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-99641
record_format dspace
spelling sg-ntu-dr.10356-996412020-06-01T10:01:33Z Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution Fan, Chunhai Zhang, Juan Wang, Lihua Zhang, Hua Boey, Freddy Yin Chiang Song, Shiping School of Materials Science & Engineering DRNTU::Engineering::Materials The design of a novel multicolor fluorescent gold nanoprobe for homogeneous detection of small-molecule targets is reported, which combines the specific binding abilities of aptamers with the ultrahigh quenching ability of gold nanoparticles (AuNPs). Dye-tagged aptamers and their complementary sequence with thiol labels are co-assembled at the surface of AuNPs. As a proof of concept, it is demonstrated that such a multicolor fluorescent gold nanoprobe can simultaneously detect adenosine, potassium ion, and cocaine with high selectivity. This potentially generic strategy is shown to be promising for rapid screening of small molecular targets. 2012-09-20T01:13:40Z 2019-12-06T20:09:46Z 2012-09-20T01:13:40Z 2019-12-06T20:09:46Z 2010 2010 Journal Article Zhang, J., Wang, L., Zhang, H., Boey, F. Y. C., Song, S., & Fan, C. (2010). Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution. Small, 6(2), 201-204. 1613-6829 https://hdl.handle.net/10356/99641 http://hdl.handle.net/10220/8584 10.1002/smll.200901012 en Small © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Engineering::Materials
spellingShingle DRNTU::Engineering::Materials
Fan, Chunhai
Zhang, Juan
Wang, Lihua
Zhang, Hua
Boey, Freddy Yin Chiang
Song, Shiping
Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution
description The design of a novel multicolor fluorescent gold nanoprobe for homogeneous detection of small-molecule targets is reported, which combines the specific binding abilities of aptamers with the ultrahigh quenching ability of gold nanoparticles (AuNPs). Dye-tagged aptamers and their complementary sequence with thiol labels are co-assembled at the surface of AuNPs. As a proof of concept, it is demonstrated that such a multicolor fluorescent gold nanoprobe can simultaneously detect adenosine, potassium ion, and cocaine with high selectivity. This potentially generic strategy is shown to be promising for rapid screening of small molecular targets.
author2 School of Materials Science & Engineering
author_facet School of Materials Science & Engineering
Fan, Chunhai
Zhang, Juan
Wang, Lihua
Zhang, Hua
Boey, Freddy Yin Chiang
Song, Shiping
format Article
author Fan, Chunhai
Zhang, Juan
Wang, Lihua
Zhang, Hua
Boey, Freddy Yin Chiang
Song, Shiping
author_sort Fan, Chunhai
title Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution
title_short Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution
title_full Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution
title_fullStr Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution
title_full_unstemmed Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution
title_sort aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution
publishDate 2012
url https://hdl.handle.net/10356/99641
http://hdl.handle.net/10220/8584
_version_ 1681056089202753536