Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution
The design of a novel multicolor fluorescent gold nanoprobe for homogeneous detection of small-molecule targets is reported, which combines the specific binding abilities of aptamers with the ultrahigh quenching ability of gold nanoparticles (AuNPs). Dye-tagged aptamers and their complementary seque...
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sg-ntu-dr.10356-996412020-06-01T10:01:33Z Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution Fan, Chunhai Zhang, Juan Wang, Lihua Zhang, Hua Boey, Freddy Yin Chiang Song, Shiping School of Materials Science & Engineering DRNTU::Engineering::Materials The design of a novel multicolor fluorescent gold nanoprobe for homogeneous detection of small-molecule targets is reported, which combines the specific binding abilities of aptamers with the ultrahigh quenching ability of gold nanoparticles (AuNPs). Dye-tagged aptamers and their complementary sequence with thiol labels are co-assembled at the surface of AuNPs. As a proof of concept, it is demonstrated that such a multicolor fluorescent gold nanoprobe can simultaneously detect adenosine, potassium ion, and cocaine with high selectivity. This potentially generic strategy is shown to be promising for rapid screening of small molecular targets. 2012-09-20T01:13:40Z 2019-12-06T20:09:46Z 2012-09-20T01:13:40Z 2019-12-06T20:09:46Z 2010 2010 Journal Article Zhang, J., Wang, L., Zhang, H., Boey, F. Y. C., Song, S., & Fan, C. (2010). Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution. Small, 6(2), 201-204. 1613-6829 https://hdl.handle.net/10356/99641 http://hdl.handle.net/10220/8584 10.1002/smll.200901012 en Small © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. |
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DRNTU::Engineering::Materials Fan, Chunhai Zhang, Juan Wang, Lihua Zhang, Hua Boey, Freddy Yin Chiang Song, Shiping Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution |
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The design of a novel multicolor fluorescent gold nanoprobe for homogeneous detection of small-molecule targets is reported, which combines the specific binding abilities of aptamers with the ultrahigh quenching ability of gold nanoparticles (AuNPs). Dye-tagged aptamers and their complementary sequence with thiol labels are co-assembled at the surface of AuNPs. As a proof of concept, it is demonstrated that such a multicolor fluorescent gold nanoprobe can simultaneously detect adenosine, potassium ion, and cocaine with high selectivity. This potentially generic strategy is shown to be promising for rapid screening of small molecular targets. |
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School of Materials Science & Engineering |
author_facet |
School of Materials Science & Engineering Fan, Chunhai Zhang, Juan Wang, Lihua Zhang, Hua Boey, Freddy Yin Chiang Song, Shiping |
format |
Article |
author |
Fan, Chunhai Zhang, Juan Wang, Lihua Zhang, Hua Boey, Freddy Yin Chiang Song, Shiping |
author_sort |
Fan, Chunhai |
title |
Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution |
title_short |
Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution |
title_full |
Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution |
title_fullStr |
Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution |
title_full_unstemmed |
Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution |
title_sort |
aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution |
publishDate |
2012 |
url |
https://hdl.handle.net/10356/99641 http://hdl.handle.net/10220/8584 |
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1681056089202753536 |