Extensive promoter-centered chromatin interactions provide a topological basis for transcription regulation

Higher-order chromosomal organization for transcription regulation is poorly understood in eukaryotes. Using genome-wide Chromatin Interaction Analysis with Paired-End-Tag sequencing (ChIA-PET), we mapped long-range chromatin interactions associated with RNA polymerase II in human cells and uncovere...

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Main Authors: Li, Guoliang, Ruan, Xiaoan, Auerbach, Raymond K., Sandhu, Kuljeet Singh, Zheng, Meizhen, Wang, Ping, Poh, Huay Mei, Goh, Yufen, Lim, Joanne, Zhang, Jingyao, Sim, Hui Shan, Peh, Su Qin, Mulawadi, Fabianus Hendriyan, Ong, Chin Thing, Orlov, Yuriy L., Hong, Shuzhen, Zhang, Zhizhuo, Landt, Steve, Raha, Debasish, Euskirchen, Ghia, Wei, Chia-Lin, Ge, Weihong, Wang, Huaien, Davis, Carrie, Fisher-Aylor, Katherine I., Mortazavi, Ali, Gerstein, Mark, Gingeras, Thomas, Wold, Barbara, Sun, Yi, Cheung, Edwin, Liu, Edison, Sung, Wing-Kin, Snyder, Michael, Ruan, Yijun, Fullwood, Melissa Jane
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2013
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Online Access:https://hdl.handle.net/10356/99700
http://hdl.handle.net/10220/10795
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-997002022-02-16T16:30:12Z Extensive promoter-centered chromatin interactions provide a topological basis for transcription regulation Li, Guoliang Ruan, Xiaoan Auerbach, Raymond K. Sandhu, Kuljeet Singh Zheng, Meizhen Wang, Ping Poh, Huay Mei Goh, Yufen Lim, Joanne Zhang, Jingyao Sim, Hui Shan Peh, Su Qin Mulawadi, Fabianus Hendriyan Ong, Chin Thing Orlov, Yuriy L. Hong, Shuzhen Zhang, Zhizhuo Landt, Steve Raha, Debasish Euskirchen, Ghia Wei, Chia-Lin Ge, Weihong Wang, Huaien Davis, Carrie Fisher-Aylor, Katherine I. Mortazavi, Ali Gerstein, Mark Gingeras, Thomas Wold, Barbara Sun, Yi Cheung, Edwin Liu, Edison Sung, Wing-Kin Snyder, Michael Ruan, Yijun Fullwood, Melissa Jane School of Biological Sciences DRNTU::Science::Biological sciences Higher-order chromosomal organization for transcription regulation is poorly understood in eukaryotes. Using genome-wide Chromatin Interaction Analysis with Paired-End-Tag sequencing (ChIA-PET), we mapped long-range chromatin interactions associated with RNA polymerase II in human cells and uncovered widespread promoter-centered intragenic, extragenic, and intergenic interactions. These interactions further aggregated into higher-order clusters, wherein proximal and distal genes were engaged through promoter-promoter interactions. Most genes with promoter-promoter interactions were active and transcribed cooperatively, and some interacting promoters could influence each other implying combinatorial complexity of transcriptional controls. Comparative analyses of different cell lines showed that cell-specific chromatin interactions could provide structural frameworks for cell-specific transcription, and suggested significant enrichment of enhancer-promoter interactions for cell-specific functions. Furthermore, genetically-identified disease-associated noncoding elements were found to be spatially engaged with corresponding genes through long-range interactions. Overall, our study provides insights into transcription regulation by three-dimensional chromatin interactions for both housekeeping and cell-specific genes in human cells. 2013-06-27T04:38:51Z 2019-12-06T20:10:31Z 2013-06-27T04:38:51Z 2019-12-06T20:10:31Z 2012 2012 Journal Article Li, G., Ruan, X., Auerbach, R. K., Sandhu, K. S., Zheng, M., Wang, P., et al. (2012). Extensive Promoter-Centered Chromatin Interactions Provide a Topological Basis for Transcription Regulation. Cell, 148(1), 84-98. 0092-8674 https://hdl.handle.net/10356/99700 http://hdl.handle.net/10220/10795 10.1016/j.cell.2011.12.014 22265404 en Cell © 2012 Elsevier Inc.
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Li, Guoliang
Ruan, Xiaoan
Auerbach, Raymond K.
Sandhu, Kuljeet Singh
Zheng, Meizhen
Wang, Ping
Poh, Huay Mei
Goh, Yufen
Lim, Joanne
Zhang, Jingyao
Sim, Hui Shan
Peh, Su Qin
Mulawadi, Fabianus Hendriyan
Ong, Chin Thing
Orlov, Yuriy L.
Hong, Shuzhen
Zhang, Zhizhuo
Landt, Steve
Raha, Debasish
Euskirchen, Ghia
Wei, Chia-Lin
Ge, Weihong
Wang, Huaien
Davis, Carrie
Fisher-Aylor, Katherine I.
Mortazavi, Ali
Gerstein, Mark
Gingeras, Thomas
Wold, Barbara
Sun, Yi
Cheung, Edwin
Liu, Edison
Sung, Wing-Kin
Snyder, Michael
Ruan, Yijun
Fullwood, Melissa Jane
Extensive promoter-centered chromatin interactions provide a topological basis for transcription regulation
description Higher-order chromosomal organization for transcription regulation is poorly understood in eukaryotes. Using genome-wide Chromatin Interaction Analysis with Paired-End-Tag sequencing (ChIA-PET), we mapped long-range chromatin interactions associated with RNA polymerase II in human cells and uncovered widespread promoter-centered intragenic, extragenic, and intergenic interactions. These interactions further aggregated into higher-order clusters, wherein proximal and distal genes were engaged through promoter-promoter interactions. Most genes with promoter-promoter interactions were active and transcribed cooperatively, and some interacting promoters could influence each other implying combinatorial complexity of transcriptional controls. Comparative analyses of different cell lines showed that cell-specific chromatin interactions could provide structural frameworks for cell-specific transcription, and suggested significant enrichment of enhancer-promoter interactions for cell-specific functions. Furthermore, genetically-identified disease-associated noncoding elements were found to be spatially engaged with corresponding genes through long-range interactions. Overall, our study provides insights into transcription regulation by three-dimensional chromatin interactions for both housekeeping and cell-specific genes in human cells.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Li, Guoliang
Ruan, Xiaoan
Auerbach, Raymond K.
Sandhu, Kuljeet Singh
Zheng, Meizhen
Wang, Ping
Poh, Huay Mei
Goh, Yufen
Lim, Joanne
Zhang, Jingyao
Sim, Hui Shan
Peh, Su Qin
Mulawadi, Fabianus Hendriyan
Ong, Chin Thing
Orlov, Yuriy L.
Hong, Shuzhen
Zhang, Zhizhuo
Landt, Steve
Raha, Debasish
Euskirchen, Ghia
Wei, Chia-Lin
Ge, Weihong
Wang, Huaien
Davis, Carrie
Fisher-Aylor, Katherine I.
Mortazavi, Ali
Gerstein, Mark
Gingeras, Thomas
Wold, Barbara
Sun, Yi
Cheung, Edwin
Liu, Edison
Sung, Wing-Kin
Snyder, Michael
Ruan, Yijun
Fullwood, Melissa Jane
format Article
author Li, Guoliang
Ruan, Xiaoan
Auerbach, Raymond K.
Sandhu, Kuljeet Singh
Zheng, Meizhen
Wang, Ping
Poh, Huay Mei
Goh, Yufen
Lim, Joanne
Zhang, Jingyao
Sim, Hui Shan
Peh, Su Qin
Mulawadi, Fabianus Hendriyan
Ong, Chin Thing
Orlov, Yuriy L.
Hong, Shuzhen
Zhang, Zhizhuo
Landt, Steve
Raha, Debasish
Euskirchen, Ghia
Wei, Chia-Lin
Ge, Weihong
Wang, Huaien
Davis, Carrie
Fisher-Aylor, Katherine I.
Mortazavi, Ali
Gerstein, Mark
Gingeras, Thomas
Wold, Barbara
Sun, Yi
Cheung, Edwin
Liu, Edison
Sung, Wing-Kin
Snyder, Michael
Ruan, Yijun
Fullwood, Melissa Jane
author_sort Li, Guoliang
title Extensive promoter-centered chromatin interactions provide a topological basis for transcription regulation
title_short Extensive promoter-centered chromatin interactions provide a topological basis for transcription regulation
title_full Extensive promoter-centered chromatin interactions provide a topological basis for transcription regulation
title_fullStr Extensive promoter-centered chromatin interactions provide a topological basis for transcription regulation
title_full_unstemmed Extensive promoter-centered chromatin interactions provide a topological basis for transcription regulation
title_sort extensive promoter-centered chromatin interactions provide a topological basis for transcription regulation
publishDate 2013
url https://hdl.handle.net/10356/99700
http://hdl.handle.net/10220/10795
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