Salting effects on protein components in aqueous NaCl and urea solutions : toward understanding of urea-induced protein denaturation

• Main Authors: Mu, Yuguang, Li, Weifeng, Zhou, Ruhong
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2013
• Subjects: DRNTU::Science::Chemistry::Analytical chemistry::Proteins
• Online Access: https://hdl.handle.net/10356/99763; http://hdl.handle.net/10220/17180
• Institution: Nanyang Technological University

The mechanism of urea-induced protein denaturation is explored through studying the salting effect of urea on 14 amino acid side chain analogues, and N-methylacetamide (NMA) which mimics the protein backbone. The solvation free energies of the 15 molecules were calculated in pure water, aqueous urea...