Real-time assessment of corneal endothelial cell damage following graft preparation and donor insertion for DMEK

• Main Authors: Bhogal M., Lwin C.N., Seah X.-Y., Murugan E., Adnan K., Lin S.-J., Peh G., Mehta J.S.
• Other Authors: DUKE-NUS MEDICAL SCHOOL
• Format: Article
• Published: 2019
• Subjects: calcein AM; fluorescent dye; unclassified drug; fluorescein derivative; adolescent; adult; aged; Article; cell viability; confocal laser scanning microscopy; controlled study; cornea transplantation; corneal endothelial cell loss; cytotoxicity; Descemet membrane endothelial keratoplasty; electron microscopy; ex vivo study; female; fluorescence; human; human cell; human tissue; in vitro study; in vivo study; intermethod comparison; major clinical study; male; measurement accuracy; middle aged; nonhuman; organ donor; porcine model; risk assessment; sensitivity and specificity; surgical wound; tissue preparation; young adult; animal; animal model; cell culture; Descemet stripping endothelial keratoplasty; donor; fluorescence microscopy; pig; procedures; Adolescent; Adult; Aged; Animals; Cells, Cultured; Corneal Endothelial Cell Loss; Descemet Stripping Endothelial Keratoplasty; Female; Fluoresceins; Humans; Male; Microscopy, Fluorescence; Middle Aged; Models, Animal; Swine; Tissue Donors; Young Adult
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/161172
• Institution: National University of Singapore

10.1371/journal.pone.0184824