Experimental Pathways towards Developing a Rotavirus Reverse Genetics System: Synthetic Full Length Rotavirus ssRNAs Are Neither Infectious nor Translated in Permissive Cells

• Main Authors: Richards J.E., Desselberger U., Lever A.M.
• Other Authors: DEPT OF MEDICINE
• Format: Article
• Published: 2019
• Subjects: double stranded RNA; enhanced green fluorescent protein; messenger RNA; nonstructural protein 2; nonstructural protein 5; single stranded RNA; article; computer model; controlled study; cytopathogenic effect; cytotoxicity; Fowlpox virus; genetic transfection; in vitro study; molecular cloning; nonhuman; protein synthesis; rabbit; reticulocyte; reverse genetics; RNA transcription; RNA translation; Rotavirus; virus genome; virus recombinant; virus strain; Animals; Blotting, Western; Cattle; Electrophoresis, Agar Gel; Genes, Viral; Polymerase Chain Reaction; Protein Biosynthesis; RNA, Viral; Transfection
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/161270
• Institution: National University of Singapore

10.1371/journal.pone.0074328