Experimental Pathways towards Developing a Rotavirus Reverse Genetics System: Synthetic Full Length Rotavirus ssRNAs Are Neither Infectious nor Translated in Permissive Cells

10.1371/journal.pone.0074328

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Main Authors:	Richards J.E., Desselberger U., Lever A.M.
Other Authors:	DEPT OF MEDICINE
Format:	Article
Published:	2019
Subjects:	double stranded RNA enhanced green fluorescent protein messenger RNA nonstructural protein 2 nonstructural protein 5 single stranded RNA article computer model controlled study cytopathogenic effect cytotoxicity Fowlpox virus genetic transfection in vitro study molecular cloning nonhuman protein synthesis rabbit reticulocyte reverse genetics RNA transcription RNA translation Rotavirus virus genome virus recombinant virus strain Animals Blotting, Western Cattle Electrophoresis, Agar Gel Genes, Viral Polymerase Chain Reaction Protein Biosynthesis RNA, Viral Transfection
Online Access:	https://scholarbank.nus.edu.sg/handle/10635/161270
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Institution:	National University of Singapore

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spelling	sg-nus-scholar.10635-1612702023-08-07T09:23:59Z Experimental Pathways towards Developing a Rotavirus Reverse Genetics System: Synthetic Full Length Rotavirus ssRNAs Are Neither Infectious nor Translated in Permissive Cells Richards J.E. Desselberger U. Lever A.M. DEPT OF MEDICINE double stranded RNA enhanced green fluorescent protein messenger RNA nonstructural protein 2 nonstructural protein 5 single stranded RNA article computer model controlled study cytopathogenic effect cytotoxicity Fowlpox virus genetic transfection in vitro study molecular cloning nonhuman protein synthesis rabbit reticulocyte reverse genetics RNA transcription RNA translation Rotavirus virus genome virus recombinant virus strain Animals Blotting, Western Cattle Electrophoresis, Agar Gel Genes, Viral Polymerase Chain Reaction Protein Biosynthesis RNA, Viral Rotavirus Transfection 10.1371/journal.pone.0074328 PLoS ONE 8 9 e74328 2019-11-04T04:02:44Z 2019-11-04T04:02:44Z 2013 Article Richards J.E., Desselberger U., Lever A.M. (2013). Experimental Pathways towards Developing a Rotavirus Reverse Genetics System: Synthetic Full Length Rotavirus ssRNAs Are Neither Infectious nor Translated in Permissive Cells. PLoS ONE 8 (9) : e74328. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0074328 19326203 https://scholarbank.nus.edu.sg/handle/10635/161270 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ Unpaywall 20191101
institution	National University of Singapore
building	NUS Library
continent	Asia
country	Singapore Singapore
content_provider	NUS Library
collection	ScholarBank@NUS
topic	double stranded RNA enhanced green fluorescent protein messenger RNA nonstructural protein 2 nonstructural protein 5 single stranded RNA article computer model controlled study cytopathogenic effect cytotoxicity Fowlpox virus genetic transfection in vitro study molecular cloning nonhuman protein synthesis rabbit reticulocyte reverse genetics RNA transcription RNA translation Rotavirus virus genome virus recombinant virus strain Animals Blotting, Western Cattle Electrophoresis, Agar Gel Genes, Viral Polymerase Chain Reaction Protein Biosynthesis RNA, Viral Rotavirus Transfection
spellingShingle	double stranded RNA enhanced green fluorescent protein messenger RNA nonstructural protein 2 nonstructural protein 5 single stranded RNA article computer model controlled study cytopathogenic effect cytotoxicity Fowlpox virus genetic transfection in vitro study molecular cloning nonhuman protein synthesis rabbit reticulocyte reverse genetics RNA transcription RNA translation Rotavirus virus genome virus recombinant virus strain Animals Blotting, Western Cattle Electrophoresis, Agar Gel Genes, Viral Polymerase Chain Reaction Protein Biosynthesis RNA, Viral Rotavirus Transfection Richards J.E. Desselberger U. Lever A.M. Experimental Pathways towards Developing a Rotavirus Reverse Genetics System: Synthetic Full Length Rotavirus ssRNAs Are Neither Infectious nor Translated in Permissive Cells
description	10.1371/journal.pone.0074328
author2	DEPT OF MEDICINE
author_facet	DEPT OF MEDICINE Richards J.E. Desselberger U. Lever A.M.
format	Article
author	Richards J.E. Desselberger U. Lever A.M.
author_sort	Richards J.E.
title	Experimental Pathways towards Developing a Rotavirus Reverse Genetics System: Synthetic Full Length Rotavirus ssRNAs Are Neither Infectious nor Translated in Permissive Cells
title_short	Experimental Pathways towards Developing a Rotavirus Reverse Genetics System: Synthetic Full Length Rotavirus ssRNAs Are Neither Infectious nor Translated in Permissive Cells
title_full	Experimental Pathways towards Developing a Rotavirus Reverse Genetics System: Synthetic Full Length Rotavirus ssRNAs Are Neither Infectious nor Translated in Permissive Cells
title_fullStr	Experimental Pathways towards Developing a Rotavirus Reverse Genetics System: Synthetic Full Length Rotavirus ssRNAs Are Neither Infectious nor Translated in Permissive Cells
title_full_unstemmed	Experimental Pathways towards Developing a Rotavirus Reverse Genetics System: Synthetic Full Length Rotavirus ssRNAs Are Neither Infectious nor Translated in Permissive Cells
title_sort	experimental pathways towards developing a rotavirus reverse genetics system: synthetic full length rotavirus ssrnas are neither infectious nor translated in permissive cells
publishDate	2019
url	https://scholarbank.nus.edu.sg/handle/10635/161270
_version_	1778169176922259456

Experimental Pathways towards Developing a Rotavirus Reverse Genetics System: Synthetic Full Length Rotavirus ssRNAs Are Neither Infectious nor Translated in Permissive Cells

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