An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells

10.1371/journal.pone.0082100

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Main Authors:	Koh E.Y.C., Ho S.C.L., Mariati Bioprocessing Technology Institute, Song Z., Bi X., Bardor M., Yang Y.
Other Authors:	BIOCHEMISTRY
Format:	Article
Published:	2019
Subjects:	monoclonal antibody myosin heavy chain myosin light chain immunoglobulin heavy chain immunoglobulin light chain animal cell article codon controlled study Encephalomyocarditis virus enzyme linked immunosorbent assay gene construct gene expression gene mutation gene vector human human cell internal ribosome entry site liquid chromatography mammal cell mass spectrometry nonhuman open reading frame polyacrylamide gel electrophoresis protein cleavage start codon supernatant translation initiation Western blotting animal cell line gel chromatography gene expression regulation genetic transfection genetics mammal metabolism mutation nucleotide sequence ribosome Animals Base Sequence Blotting, Western Cell Line Chromatography, Gel Gene Expression Regulation Humans Immunoglobulin Heavy Chains Immunoglobulin Light Chains Mammals Mutation Ribosomes Transfection Gene Expression Gene Order Open Reading Frames Recombinant Fusion Proteins Repetitive Sequences, Nucleic Acid RNA RNA, Long Noncoding
Online Access:	https://scholarbank.nus.edu.sg/handle/10635/161448
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Institution:	National University of Singapore

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spelling	sg-nus-scholar.10635-1614482024-04-22T03:11:30Z An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells Koh E.Y.C. Ho S.C.L. Mariati Bioprocessing Technology Institute Song Z. Bi X. Bardor M. Yang Y. BIOCHEMISTRY monoclonal antibody myosin heavy chain myosin light chain immunoglobulin heavy chain immunoglobulin light chain animal cell article codon controlled study Encephalomyocarditis virus enzyme linked immunosorbent assay gene construct gene expression gene mutation gene vector human human cell internal ribosome entry site liquid chromatography mammal cell mass spectrometry nonhuman open reading frame polyacrylamide gel electrophoresis protein cleavage start codon supernatant translation initiation Western blotting animal cell line gel chromatography gene expression regulation genetic transfection genetics mammal metabolism mutation nucleotide sequence ribosome Animals Base Sequence Blotting, Western Cell Line Chromatography, Gel Encephalomyocarditis virus Gene Expression Regulation Humans Immunoglobulin Heavy Chains Immunoglobulin Light Chains Mammals Mutation Ribosomes Transfection Cell Line Gene Expression Gene Order Humans Open Reading Frames Recombinant Fusion Proteins Repetitive Sequences, Nucleic Acid RNA RNA, Long Noncoding 10.1371/journal.pone.0082100 PLoS ONE 8 12 e82100 2019-11-05T02:07:56Z 2019-11-05T02:07:56Z 2013 Article Koh E.Y.C., Ho S.C.L., Mariati Bioprocessing Technology Institute, Song Z., Bi X., Bardor M., Yang Y. (2013). An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells. PLoS ONE 8 (12) : e82100. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0082100 1932-6203 https://scholarbank.nus.edu.sg/handle/10635/161448 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ Unpaywall 20191101
institution	National University of Singapore
building	NUS Library
continent	Asia
country	Singapore Singapore
content_provider	NUS Library
collection	ScholarBank@NUS
topic	monoclonal antibody myosin heavy chain myosin light chain immunoglobulin heavy chain immunoglobulin light chain animal cell article codon controlled study Encephalomyocarditis virus enzyme linked immunosorbent assay gene construct gene expression gene mutation gene vector human human cell internal ribosome entry site liquid chromatography mammal cell mass spectrometry nonhuman open reading frame polyacrylamide gel electrophoresis protein cleavage start codon supernatant translation initiation Western blotting animal cell line gel chromatography gene expression regulation genetic transfection genetics mammal metabolism mutation nucleotide sequence ribosome Animals Base Sequence Blotting, Western Cell Line Chromatography, Gel Encephalomyocarditis virus Gene Expression Regulation Humans Immunoglobulin Heavy Chains Immunoglobulin Light Chains Mammals Mutation Ribosomes Transfection Cell Line Gene Expression Gene Order Humans Open Reading Frames Recombinant Fusion Proteins Repetitive Sequences, Nucleic Acid RNA RNA, Long Noncoding
spellingShingle	monoclonal antibody myosin heavy chain myosin light chain immunoglobulin heavy chain immunoglobulin light chain animal cell article codon controlled study Encephalomyocarditis virus enzyme linked immunosorbent assay gene construct gene expression gene mutation gene vector human human cell internal ribosome entry site liquid chromatography mammal cell mass spectrometry nonhuman open reading frame polyacrylamide gel electrophoresis protein cleavage start codon supernatant translation initiation Western blotting animal cell line gel chromatography gene expression regulation genetic transfection genetics mammal metabolism mutation nucleotide sequence ribosome Animals Base Sequence Blotting, Western Cell Line Chromatography, Gel Encephalomyocarditis virus Gene Expression Regulation Humans Immunoglobulin Heavy Chains Immunoglobulin Light Chains Mammals Mutation Ribosomes Transfection Cell Line Gene Expression Gene Order Humans Open Reading Frames Recombinant Fusion Proteins Repetitive Sequences, Nucleic Acid RNA RNA, Long Noncoding Koh E.Y.C. Ho S.C.L. Mariati Bioprocessing Technology Institute Song Z. Bi X. Bardor M. Yang Y. An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells
description	10.1371/journal.pone.0082100
author2	BIOCHEMISTRY
author_facet	BIOCHEMISTRY Koh E.Y.C. Ho S.C.L. Mariati Bioprocessing Technology Institute Song Z. Bi X. Bardor M. Yang Y.
format	Article
author	Koh E.Y.C. Ho S.C.L. Mariati Bioprocessing Technology Institute Song Z. Bi X. Bardor M. Yang Y.
author_sort	Koh E.Y.C.
title	An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells
title_short	An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells
title_full	An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells
title_fullStr	An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells
title_full_unstemmed	An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells
title_sort	internal ribosome entry site (ires) mutant library for tuning expression level of multiple genes in mammalian cells
publishDate	2019
url	https://scholarbank.nus.edu.sg/handle/10635/161448
_version_	1800913795001876480

An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells

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