An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells

• Main Authors: Koh E.Y.C., Ho S.C.L., Mariati Bioprocessing Technology Institute, Song Z., Bi X., Bardor M., Yang Y.
• Other Authors: BIOCHEMISTRY
• Format: Article
• Published: 2019
• Subjects: monoclonal antibody; myosin heavy chain; myosin light chain; immunoglobulin heavy chain; immunoglobulin light chain; animal cell; article; codon; controlled study; Encephalomyocarditis virus; enzyme linked immunosorbent assay; gene construct; gene expression; gene mutation; gene vector; human; human cell; internal ribosome entry site; liquid chromatography; mammal cell; mass spectrometry; nonhuman; open reading frame; polyacrylamide gel electrophoresis; protein cleavage; start codon; supernatant; translation initiation; Western blotting; animal; cell line; gel chromatography; gene expression regulation; genetic transfection; genetics; mammal; metabolism; mutation; nucleotide sequence; ribosome; Animals; Base Sequence; Blotting, Western; Cell Line; Chromatography, Gel; Gene Expression Regulation; Humans; Immunoglobulin Heavy Chains; Immunoglobulin Light Chains; Mammals; Mutation; Ribosomes; Transfection; Gene Expression; Gene Order; Open Reading Frames; Recombinant Fusion Proteins; Repetitive Sequences, Nucleic Acid; RNA; RNA, Long Noncoding
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/161448
• Institution: National University of Singapore

10.1371/journal.pone.0082100