Rational Design of a Live Attenuated Dengue Vaccine: 2?-O-Methyltransferase Mutants Are Highly Attenuated and Immunogenic in Mice and Macaques

• Main Authors: Züst R., Dong H., Li X.-F., Chang D.C., Zhang B., Balakrishnan T., Toh Y.-X., Jiang T., Li S.-H., Deng Y.-Q., Ellis B.R., Ellis E.M., Poidinger M., Zolezzi F., Qin C.-F., Shi P.-Y., Fink K.
• Other Authors: BIOLOGICAL SCIENCES
• Format: Article
• Published: 2019
• Subjects: 2 O methyltransferase; dengue vaccine; immunoglobulin G; interferon; neutralizing antibody; unclassified drug; virus envelope protein; virus enzyme; virus RNA; live vaccine; methyltransferase; RNA 2'-O-methyltransferase; adaptive immunity; animal experiment; animal model; animal tissue; antibody dependent enhancement; article; CD8+ T lymphocyte; cell strain BHK; cell strain HEK293; controlled study; dengue; Dengue virus 1; Dengue virus 2; enzyme linked immunosorbent assay; female; flow cytometry; human; human cell; immunofluorescence; innate immunity; male; methylation; mosquito; mouse; nonhuman; reverse transcription polymerase chain reaction; rhesus monkey; seroconversion; viremia; virus isolation; virus mutant; virus neutralization; virus transmission; animal; Dengue virus; enzymology; genetics; hamster; HEK293 cell line; immunology; mutant mouse strain; mutation; Animals; Cricetinae; Dengue; Dengue Vaccines; Dengue Virus; HEK293 Cells; Humans; Interferon Type I; Macaca mulatta; Methyltransferases; Mice; Mice, Mutant Strains; Mutation; Vaccines, Attenuated
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/161619
• Institution: National University of Singapore

10.1371/journal.ppat.1003521