Optimization of heavy chain and light chain signal peptides for high level expression of therapeutic antibodies in CHO cells

• Main Authors: Haryadi R., Ho S., Kok Y.J., Pu H.X., Zheng L., Pereira N.A., Li B., Bi X., Goh L.-T., Yang Y., Song Z.
• Other Authors: BIOCHEMISTRY
• Format: Article
• Published: 2019
• Subjects: adalimumab; aspartic acid; aspartylyisoleucylglutaminylmethionylthreonylglutaminylserylprolylserylserylleucylserylalanylserylvalylglycylaspartylarginine; bevacizumab; cysteine; glutamic acid; glutamylserylglycylglycylglycylleucylvalylglutaminylprolylglycylglycylserylleucylarginine; glutamylvalylglutaminylleucylvalylglutamylserylglycylglycylglycylvalylglutaminylprolylglycylglycylserylleucylarginine; immunoglobulin kappa chain; immunoglobulin light chain; infliximab; methionylaspartylphenyalalyglutaminylvalylisoleucylisoleucylserylphenylalanylleucylleucylisoleucylserylalalylserylvalylisoleucylmethionylserylarginylglycine; methionylglutamylphenylalanylglycylleucylseryltryptophanylvalylphenylalanylleucylvalylalanylleucylphenylalanylarginylglycylvalylglutamylcysteine; methionylglyclytryptophanylserylleucylisoleucylleucylleucylphenylalanylleucylvalylalanylvalylalanyltyrosylarginylvalylleucylserine; peptide; rituximab; trastuzumab; unclassified drug; immunoglobulin G; immunoglobulin heavy chain; monoclonal antibody; signal peptide; amino acid sequence; amino acid substitution; animal cell; antibody detection; antibody production; antibody titer; antigen expression; Article; mass spectrometry; nonhuman; peptide analysis; phylogeny; protein cleavage; protein processing; sequence alignment; sequence homology; animal; CHO cell line; Cricetulus; metabolism; physiology; Animals; Antibodies, Monoclonal; CHO Cells; Immunoglobulin G; Immunoglobulin Heavy Chains; Immunoglobulin Light Chains; Mass Spectrometry; Protein Sorting Signals
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/161742
• Institution: National University of Singapore

10.1371/journal.pone.0116878