Optimization of heavy chain and light chain signal peptides for high level expression of therapeutic antibodies in CHO cells

10.1371/journal.pone.0116878

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Main Authors:	Haryadi R., Ho S., Kok Y.J., Pu H.X., Zheng L., Pereira N.A., Li B., Bi X., Goh L.-T., Yang Y., Song Z.
Other Authors:	BIOCHEMISTRY
Format:	Article
Published:	2019
Subjects:	adalimumab aspartic acid aspartylyisoleucylglutaminylmethionylthreonylglutaminylserylprolylserylserylleucylserylalanylserylvalylglycylaspartylarginine bevacizumab cysteine glutamic acid glutamylserylglycylglycylglycylleucylvalylglutaminylprolylglycylglycylserylleucylarginine glutamylvalylglutaminylleucylvalylglutamylserylglycylglycylglycylvalylglutaminylprolylglycylglycylserylleucylarginine immunoglobulin kappa chain immunoglobulin light chain infliximab methionylaspartylphenyalalyglutaminylvalylisoleucylisoleucylserylphenylalanylleucylleucylisoleucylserylalalylserylvalylisoleucylmethionylserylarginylglycine methionylglutamylphenylalanylglycylleucylseryltryptophanylvalylphenylalanylleucylvalylalanylleucylphenylalanylarginylglycylvalylglutamylcysteine methionylglyclytryptophanylserylleucylisoleucylleucylleucylphenylalanylleucylvalylalanylvalylalanyltyrosylarginylvalylleucylserine peptide rituximab trastuzumab unclassified drug immunoglobulin G immunoglobulin heavy chain monoclonal antibody signal peptide amino acid sequence amino acid substitution animal cell antibody detection antibody production antibody titer antigen expression Article mass spectrometry nonhuman peptide analysis phylogeny protein cleavage protein processing sequence alignment sequence homology animal CHO cell line Cricetulus metabolism physiology Animals Antibodies, Monoclonal CHO Cells Immunoglobulin G Immunoglobulin Heavy Chains Immunoglobulin Light Chains Mass Spectrometry Protein Sorting Signals
Online Access:	https://scholarbank.nus.edu.sg/handle/10635/161742
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Institution:	National University of Singapore

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spelling	sg-nus-scholar.10635-1617422024-04-22T03:01:18Z Optimization of heavy chain and light chain signal peptides for high level expression of therapeutic antibodies in CHO cells Haryadi R. Ho S. Kok Y.J. Pu H.X. Zheng L. Pereira N.A. Li B. Bi X. Goh L.-T. Yang Y. Song Z. BIOCHEMISTRY adalimumab aspartic acid aspartylyisoleucylglutaminylmethionylthreonylglutaminylserylprolylserylserylleucylserylalanylserylvalylglycylaspartylarginine bevacizumab cysteine glutamic acid glutamylserylglycylglycylglycylleucylvalylglutaminylprolylglycylglycylserylleucylarginine glutamylvalylglutaminylleucylvalylglutamylserylglycylglycylglycylvalylglutaminylprolylglycylglycylserylleucylarginine immunoglobulin kappa chain immunoglobulin light chain infliximab methionylaspartylphenyalalyglutaminylvalylisoleucylisoleucylserylphenylalanylleucylleucylisoleucylserylalalylserylvalylisoleucylmethionylserylarginylglycine methionylglutamylphenylalanylglycylleucylseryltryptophanylvalylphenylalanylleucylvalylalanylleucylphenylalanylarginylglycylvalylglutamylcysteine methionylglyclytryptophanylserylleucylisoleucylleucylleucylphenylalanylleucylvalylalanylvalylalanyltyrosylarginylvalylleucylserine peptide rituximab trastuzumab unclassified drug immunoglobulin G immunoglobulin heavy chain immunoglobulin light chain monoclonal antibody signal peptide amino acid sequence amino acid substitution animal cell antibody detection antibody production antibody titer antigen expression Article mass spectrometry nonhuman peptide analysis phylogeny protein cleavage protein processing sequence alignment sequence homology animal CHO cell line Cricetulus metabolism physiology Animals Antibodies, Monoclonal CHO Cells Cricetulus Immunoglobulin G Immunoglobulin Heavy Chains Immunoglobulin Light Chains Mass Spectrometry Protein Sorting Signals 10.1371/journal.pone.0116878 PLoS ONE 10 2 e0116878 2019-11-07T05:01:17Z 2019-11-07T05:01:17Z 2015 Article Haryadi R., Ho S., Kok Y.J., Pu H.X., Zheng L., Pereira N.A., Li B., Bi X., Goh L.-T., Yang Y., Song Z. (2015). Optimization of heavy chain and light chain signal peptides for high level expression of therapeutic antibodies in CHO cells. PLoS ONE 10 (2) : e0116878. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0116878 19326203 https://scholarbank.nus.edu.sg/handle/10635/161742 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ Unpaywall 20191101
institution	National University of Singapore
building	NUS Library
continent	Asia
country	Singapore Singapore
content_provider	NUS Library
collection	ScholarBank@NUS
topic	adalimumab aspartic acid aspartylyisoleucylglutaminylmethionylthreonylglutaminylserylprolylserylserylleucylserylalanylserylvalylglycylaspartylarginine bevacizumab cysteine glutamic acid glutamylserylglycylglycylglycylleucylvalylglutaminylprolylglycylglycylserylleucylarginine glutamylvalylglutaminylleucylvalylglutamylserylglycylglycylglycylvalylglutaminylprolylglycylglycylserylleucylarginine immunoglobulin kappa chain immunoglobulin light chain infliximab methionylaspartylphenyalalyglutaminylvalylisoleucylisoleucylserylphenylalanylleucylleucylisoleucylserylalalylserylvalylisoleucylmethionylserylarginylglycine methionylglutamylphenylalanylglycylleucylseryltryptophanylvalylphenylalanylleucylvalylalanylleucylphenylalanylarginylglycylvalylglutamylcysteine methionylglyclytryptophanylserylleucylisoleucylleucylleucylphenylalanylleucylvalylalanylvalylalanyltyrosylarginylvalylleucylserine peptide rituximab trastuzumab unclassified drug immunoglobulin G immunoglobulin heavy chain immunoglobulin light chain monoclonal antibody signal peptide amino acid sequence amino acid substitution animal cell antibody detection antibody production antibody titer antigen expression Article mass spectrometry nonhuman peptide analysis phylogeny protein cleavage protein processing sequence alignment sequence homology animal CHO cell line Cricetulus metabolism physiology Animals Antibodies, Monoclonal CHO Cells Cricetulus Immunoglobulin G Immunoglobulin Heavy Chains Immunoglobulin Light Chains Mass Spectrometry Protein Sorting Signals
spellingShingle	adalimumab aspartic acid aspartylyisoleucylglutaminylmethionylthreonylglutaminylserylprolylserylserylleucylserylalanylserylvalylglycylaspartylarginine bevacizumab cysteine glutamic acid glutamylserylglycylglycylglycylleucylvalylglutaminylprolylglycylglycylserylleucylarginine glutamylvalylglutaminylleucylvalylglutamylserylglycylglycylglycylvalylglutaminylprolylglycylglycylserylleucylarginine immunoglobulin kappa chain immunoglobulin light chain infliximab methionylaspartylphenyalalyglutaminylvalylisoleucylisoleucylserylphenylalanylleucylleucylisoleucylserylalalylserylvalylisoleucylmethionylserylarginylglycine methionylglutamylphenylalanylglycylleucylseryltryptophanylvalylphenylalanylleucylvalylalanylleucylphenylalanylarginylglycylvalylglutamylcysteine methionylglyclytryptophanylserylleucylisoleucylleucylleucylphenylalanylleucylvalylalanylvalylalanyltyrosylarginylvalylleucylserine peptide rituximab trastuzumab unclassified drug immunoglobulin G immunoglobulin heavy chain immunoglobulin light chain monoclonal antibody signal peptide amino acid sequence amino acid substitution animal cell antibody detection antibody production antibody titer antigen expression Article mass spectrometry nonhuman peptide analysis phylogeny protein cleavage protein processing sequence alignment sequence homology animal CHO cell line Cricetulus metabolism physiology Animals Antibodies, Monoclonal CHO Cells Cricetulus Immunoglobulin G Immunoglobulin Heavy Chains Immunoglobulin Light Chains Mass Spectrometry Protein Sorting Signals Haryadi R. Ho S. Kok Y.J. Pu H.X. Zheng L. Pereira N.A. Li B. Bi X. Goh L.-T. Yang Y. Song Z. Optimization of heavy chain and light chain signal peptides for high level expression of therapeutic antibodies in CHO cells
description	10.1371/journal.pone.0116878
author2	BIOCHEMISTRY
author_facet	BIOCHEMISTRY Haryadi R. Ho S. Kok Y.J. Pu H.X. Zheng L. Pereira N.A. Li B. Bi X. Goh L.-T. Yang Y. Song Z.
format	Article
author	Haryadi R. Ho S. Kok Y.J. Pu H.X. Zheng L. Pereira N.A. Li B. Bi X. Goh L.-T. Yang Y. Song Z.
author_sort	Haryadi R.
title	Optimization of heavy chain and light chain signal peptides for high level expression of therapeutic antibodies in CHO cells
title_short	Optimization of heavy chain and light chain signal peptides for high level expression of therapeutic antibodies in CHO cells
title_full	Optimization of heavy chain and light chain signal peptides for high level expression of therapeutic antibodies in CHO cells
title_fullStr	Optimization of heavy chain and light chain signal peptides for high level expression of therapeutic antibodies in CHO cells
title_full_unstemmed	Optimization of heavy chain and light chain signal peptides for high level expression of therapeutic antibodies in CHO cells
title_sort	optimization of heavy chain and light chain signal peptides for high level expression of therapeutic antibodies in cho cells
publishDate	2019
url	https://scholarbank.nus.edu.sg/handle/10635/161742
_version_	1800913807671820288

Optimization of heavy chain and light chain signal peptides for high level expression of therapeutic antibodies in CHO cells

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