Nonvirally modified autologous primary hepatocytes correct diabetes and prevent target organ injury in a large preclinical model

• Main Authors: Chen N.K.F., Wong J.S., Kee I.H.C., Lai S.H., Thng C.H., Ng W.H., Ng R.T.H., Tan S.Y., Lee S.Y., Tan M.E.H., Sivalingam J., Chow P.K.H., Kon O.L.
• Other Authors: DUKE-NUS MEDICAL SCHOOL
• Format: Article
• Published: 2019
• Subjects: C peptide; complementary DNA; glucose; insulin; messenger RNA; transcriptome; streptozocin; animal cell; animal experiment; animal model; animal tissue; aorta; article; cell isolation; cell transplantation; controlled study; diabetes mellitus; dyslipidemia; electroporation; ex vivo study; expression vector; eye; female; gene transfer; glucose blood level; glucose intolerance; hyperglycemia; kidney; liver; liver cell; liver function; male; metabolic disorder; nonhuman; organ injury; plasmid; streptozocin diabetes; swine; animal; DNA microarray; experimental diabetes mellitus; gene expression profiling; gene therapy; genetics; glucose tolerance test; human; injury; metabolism; pancreas islet beta cell; pathology; radioimmunoassay; retina; reverse transcription polymerase chain reaction; secretion; transplantation; Suidae; Animals; Aorta; Blood Glucose; C-Peptide; Diabetes Mellitus, Experimental; Electroporation; Gene Expression Profiling; Gene Therapy; Gene Transfer Techniques; Glucose Tolerance Test; Hepatocytes; Humans; Insulin; Insulin-Secreting Cells; Kidney; Liver; Oligonucleotide Array Sequence Analysis; Radioimmunoassay; Retina; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Streptozocin; Swine
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/161858
• Institution: National University of Singapore

10.1371/journal.pone.0001734