Differences in AMY1 gene copy numbers derived from blood, buccal cells and saliva using quantitative and droplet digital PCR methods: Flagging the pitfall

• Main Authors: Ooi D.S.Q., Tan V.M.H., Ong S.G., Chan Y.H., Heng C.K., Lee Y.S.
• Other Authors: DEAN'S OFFICE (MEDICINE)
• Format: Article
• Published: Public Library of Science 2020
• Subjects: adult; AMY1 gene; Article; blood sampling; body mass; cheek cell; controlled study; droplet digital polymerase chain reaction; gene; gene dosage; human; informed consent; limit of quantitation; male; polymerase chain reaction; questionnaire; real time polymerase chain reaction; saliva analysis; smoking; adolescent; chemistry; copy number variation; gene expression; genetics; isolation and purification; metabolism; middle aged; mononuclear cell; mouth mucosa; observer variation; procedures; reproducibility; saliva; standards; alpha amylase saliva isoenzyme; AMY1A protein, human; DNA; Adolescent; Adult; DNA Copy Number Variations; Gene Dosage; Gene Expression; Humans; Leukocytes, Mononuclear; Male; Middle Aged; Mouth Mucosa; Observer Variation; Polymerase Chain Reaction; Reproducibility of Results; Saliva; Salivary alpha-Amylases
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/166025
• Institution: National University of Singapore

10.1371/journal.pone.0170767