Differences in AMY1 gene copy numbers derived from blood, buccal cells and saliva using quantitative and droplet digital PCR methods: Flagging the pitfall

10.1371/journal.pone.0170767

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Main Authors: Ooi D.S.Q., Tan V.M.H., Ong S.G., Chan Y.H., Heng C.K., Lee Y.S.
Other Authors: DEAN'S OFFICE (MEDICINE)
Format: Article
Published: Public Library of Science 2020
Subjects:
DNA
Online Access:https://scholarbank.nus.edu.sg/handle/10635/166025
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spelling sg-nus-scholar.10635-1660252024-11-14T06:49:52Z Differences in AMY1 gene copy numbers derived from blood, buccal cells and saliva using quantitative and droplet digital PCR methods: Flagging the pitfall Ooi D.S.Q. Tan V.M.H. Ong S.G. Chan Y.H. Heng C.K. Lee Y.S. DEAN'S OFFICE (MEDICINE) PAEDIATRICS adult AMY1 gene Article blood sampling body mass cheek cell controlled study droplet digital polymerase chain reaction gene gene dosage human informed consent limit of quantitation male polymerase chain reaction questionnaire real time polymerase chain reaction saliva analysis smoking adolescent chemistry copy number variation gene expression genetics isolation and purification metabolism middle aged mononuclear cell mouth mucosa observer variation polymerase chain reaction procedures reproducibility saliva standards alpha amylase saliva isoenzyme AMY1A protein, human DNA Adolescent Adult DNA DNA Copy Number Variations Gene Dosage Gene Expression Humans Leukocytes, Mononuclear Male Middle Aged Mouth Mucosa Observer Variation Polymerase Chain Reaction Reproducibility of Results Saliva Salivary alpha-Amylases 10.1371/journal.pone.0170767 PLoS ONE 12 1 e0170767 2020-03-27T06:28:35Z 2020-03-27T06:28:35Z 2017 Article Ooi D.S.Q., Tan V.M.H., Ong S.G., Chan Y.H., Heng C.K., Lee Y.S. (2017). Differences in AMY1 gene copy numbers derived from blood, buccal cells and saliva using quantitative and droplet digital PCR methods: Flagging the pitfall. PLoS ONE 12 (1) : e0170767. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0170767 19326203 https://scholarbank.nus.edu.sg/handle/10635/166025 Public Library of Science Unpaywall 20200320
institution National University of Singapore
building NUS Library
continent Asia
country Singapore
Singapore
content_provider NUS Library
collection ScholarBank@NUS
topic adult
AMY1 gene
Article
blood sampling
body mass
cheek cell
controlled study
droplet digital polymerase chain reaction
gene
gene dosage
human
informed consent
limit of quantitation
male
polymerase chain reaction
questionnaire
real time polymerase chain reaction
saliva analysis
smoking
adolescent
chemistry
copy number variation
gene expression
genetics
isolation and purification
metabolism
middle aged
mononuclear cell
mouth mucosa
observer variation
polymerase chain reaction
procedures
reproducibility
saliva
standards
alpha amylase saliva isoenzyme
AMY1A protein, human
DNA
Adolescent
Adult
DNA
DNA Copy Number Variations
Gene Dosage
Gene Expression
Humans
Leukocytes, Mononuclear
Male
Middle Aged
Mouth Mucosa
Observer Variation
Polymerase Chain Reaction
Reproducibility of Results
Saliva
Salivary alpha-Amylases
spellingShingle adult
AMY1 gene
Article
blood sampling
body mass
cheek cell
controlled study
droplet digital polymerase chain reaction
gene
gene dosage
human
informed consent
limit of quantitation
male
polymerase chain reaction
questionnaire
real time polymerase chain reaction
saliva analysis
smoking
adolescent
chemistry
copy number variation
gene expression
genetics
isolation and purification
metabolism
middle aged
mononuclear cell
mouth mucosa
observer variation
polymerase chain reaction
procedures
reproducibility
saliva
standards
alpha amylase saliva isoenzyme
AMY1A protein, human
DNA
Adolescent
Adult
DNA
DNA Copy Number Variations
Gene Dosage
Gene Expression
Humans
Leukocytes, Mononuclear
Male
Middle Aged
Mouth Mucosa
Observer Variation
Polymerase Chain Reaction
Reproducibility of Results
Saliva
Salivary alpha-Amylases
Ooi D.S.Q.
Tan V.M.H.
Ong S.G.
Chan Y.H.
Heng C.K.
Lee Y.S.
Differences in AMY1 gene copy numbers derived from blood, buccal cells and saliva using quantitative and droplet digital PCR methods: Flagging the pitfall
description 10.1371/journal.pone.0170767
author2 DEAN'S OFFICE (MEDICINE)
author_facet DEAN'S OFFICE (MEDICINE)
Ooi D.S.Q.
Tan V.M.H.
Ong S.G.
Chan Y.H.
Heng C.K.
Lee Y.S.
format Article
author Ooi D.S.Q.
Tan V.M.H.
Ong S.G.
Chan Y.H.
Heng C.K.
Lee Y.S.
author_sort Ooi D.S.Q.
title Differences in AMY1 gene copy numbers derived from blood, buccal cells and saliva using quantitative and droplet digital PCR methods: Flagging the pitfall
title_short Differences in AMY1 gene copy numbers derived from blood, buccal cells and saliva using quantitative and droplet digital PCR methods: Flagging the pitfall
title_full Differences in AMY1 gene copy numbers derived from blood, buccal cells and saliva using quantitative and droplet digital PCR methods: Flagging the pitfall
title_fullStr Differences in AMY1 gene copy numbers derived from blood, buccal cells and saliva using quantitative and droplet digital PCR methods: Flagging the pitfall
title_full_unstemmed Differences in AMY1 gene copy numbers derived from blood, buccal cells and saliva using quantitative and droplet digital PCR methods: Flagging the pitfall
title_sort differences in amy1 gene copy numbers derived from blood, buccal cells and saliva using quantitative and droplet digital pcr methods: flagging the pitfall
publisher Public Library of Science
publishDate 2020
url https://scholarbank.nus.edu.sg/handle/10635/166025
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