Suppressive effect of secretory phospholipase A2inhibitory peptide on interleukin-1β-induced matrix metalloproteinase production in rheumatoid synovial fibroblasts, and its antiarthritic activity in hTNFtg mice

• Main Authors: Thwin M.-M., Douni E., Arjunan P., Kollias G., Kumar P.V., Gopalakrishnakone P.
• Other Authors: ANATOMY
• Format: Article
• Published: BioMed Central Ltd. 2020
• Subjects: antiflamin 2; antirheumatic agent; celecoxib; gelatinase A; gelatinase B; infliximab; interleukin 1beta; interleukin 6; interstitial collagenase; matrix metalloproteinase; matrix metalloproteinase inhibitor; messenger RNA; methotrexate; mitogen activated protein kinase p38; phospholipase A2 group II; phospholipase A2 group IIA; phospholipase A2 inhibitor; phospholipase inhibitor from python 18; stromelysin; synthetic peptide; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinase 2; tumor necrosis factor; tumor necrosis factor alpha; unclassified drug; varespladib; enzyme inhibitor; peptide; secretory phospholipase A2; animal cell; animal experiment; animal model; animal tissue; ankle; article; cell stimulation; concentration response; controlled study; drug effect; drug efficacy; drug mechanism; enzyme activity; enzyme inhibition; enzyme linked immunosorbent assay; enzyme phosphorylation; enzyme synthesis; fibroblast culture; gene expression; histopathology; human; human cell; human tissue; mouse; nonhuman; osteoarthritis; protein expression; real time polymerase chain reaction; rheumatoid arthritis; RNA transcription; synoviocyte; animal; biosynthesis; cytology; drug antagonism; fibroblast; genetic transcription; genetics; metabolism; reverse transcription polymerase chain reaction; synovium; transgenic mouse; Animals; Arthritis, Rheumatoid; Enzyme Inhibitors; Enzyme-Linked Immunosorbent Assay; Fibroblasts; Gene Expression; Humans; Interleukin-1beta; Matrix Metalloproteinases; Mice; Mice, Transgenic; Peptides; Phospholipases A2, Secretory; Reverse Transcriptase Polymerase Chain Reaction; Synovial Membrane; Transcription, Genetic; Tumor Necrosis Factor-alpha
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/174461
• Institution: National University of Singapore

10.1186/ar2810