Efficient genome editing using CRISPR/Cas9 ribonucleoprotein approach in cultured Medaka fish cells

• Main Authors: Liu, Q, Yuan, Y, Zhu, F, Hong, Y, Ge, R
• Other Authors: BIOLOGICAL SCIENCES
• Format: Article
• Published: 2020
• Subjects: cas9 protein; cellular apoptosis susceptibility protein; CRISPR associated protein; DNA; guide RNA; heteroduplex; ntrk3b protein; protein tyrosine kinase; ribonucleoprotein; unclassified drug; animal cell; Article; cell assay; cell culture; clonal cell line; controlled study; CRISPR-CAS9 system; diploidy; DNA end joining repair; DNA sequence; electroporation; gene editing; gene expression; gene function; gene mutation; gene targeting; genetic transfection; haploidy; in vitro study; nonhuman; Oryzias latipes; polyacrylamide gel electrophoresis; polymerase chain reaction; protein synthesis
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/175074
• Institution: National University of Singapore

10.1242/bio.035170