Development of plate reader and on-line microfluidic screening to identify ligands of the 5-hydroxytryptamine binding protein in venoms

• Main Authors: Otvos, R.A, Iyer, J.K, van Elk, R, Ulens, C, Niessen, W.M.A, Somsen, G.W, Manjunatha Kini, R, Smit, A.B, Kool, J
• Other Authors: BIOLOGICAL SCIENCES
• Format: Article
• Published: 2020
• Subjects: binding protein; granisetron; ligand; recombinant protein; serotonin; serotonin binding protein; serotonin receptor; snake venom; trypsin; unclassified drug; nicotinic receptor; protein binding; proteome; reptilian protein; serotonin 3 receptor; amino acid sequence; analytical equipment; Aplysia californica; Article; Dendroapsis polylepis; electrospray mass spectrometry; fluorescence analysis; fluorometer; gel permeation chromatography; gene expression system; high performance liquid chromatography; hydrolysis; IC50; mass spectrometer; microfluidic analysis; microplate; microplate reader; nonhuman; protein analysis; protein purification; proteomics; Pseudonaja affinis; Pseudonaja inframacula; radioassay; rapid separation liquid chromatography column; receptor binding assay; snake; ultra performance liquid chromatography; binding site; chemistry; devices; genetics; isolation and purification; lab on a chip; online system; procedures; protein engineering; Polylepis; Serpentes; Binding Sites; Lab-On-A-Chip Devices; Ligands; Microfluidic Analytical Techniques; Online Systems; Protein Binding; Protein Engineering; Proteome; Radioligand Assay; Receptors, Nicotinic; Receptors, Serotonin, 5-HT3; Reptilian Proteins; Snake Venoms
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/175993
• Institution: National University of Singapore

10.3390/toxins7072336