A thiol probe for measuring unfolded protein load and proteostasis in cells

• Main Authors: Chen, M.Z, Moily, N.S, Bridgford, J.L, Wood, R.J, Radwan, M, Smith, T.A, Song, Z, Tang, B.Z, Tilley, L, Xu, X, Reid, G.E, Pouladi, M.A, Hong, Y, Hatters, D.M
• Other Authors: MEDICINE
• Format: Article
• Published: Nature Publishing Group 2020
• Subjects: beta lactoglobulin; cysteine; dihydroartemisinin; dye; enolase; globular protein; glutathione; huntingtin; peroxiredoxin 3; protein; proteome; tetraphenylethene maleimide; thiol; ubiquitin; unclassified drug; artemisinin derivative; epoxomicin; fluorescent dye; maleimide; maleimide derivative; mutant protein; oligopeptide; thiol derivative; tunicamycin; cells and cell components; nervous system disorder; parasite; proteomics; reaction kinetics; Article; exon; human; Huntington chorea; induced pluripotent stem cell; nonhuman; Plasmodium falciparum; protein folding; protein homeostasis; protein unfolding; animal; cells; chemistry; drug effects; HEK293 cell line; HeLa cell line; malaria; metabolism; molecular probe; mouse; parasitology; solubility; Animals; Artemisinins; Cells; Cysteine; Fluorescent Dyes; HEK293 Cells; HeLa Cells; Humans; Huntingtin Protein; Malaria; Maleimides; Mice; Molecular Probes; Mutant Proteins; Oligopeptides; Parasites; Protein Folding; Proteome; Proteostasis; Solubility; Sulfhydryl Compounds; Tunicamycin
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/178580
• Institution: National University of Singapore

10.1038/s41467-017-00203-5