Characterization of H type 1 and type 1 N-Acetyllactosamine glycan epitopes on ovarian cancer specifically recognized by the anti-glycan monoclonal antibody mAb-A4

• Main Authors: Choo, M, Tan, H.L, Ding, V, Belgacem, O, Liau, B, Hartley-Tassell, L, Haslam, S.M, Dell, A, Choo, A
• Other Authors: BIOMEDICAL ENGINEERING
• Format: Article
• Published: American Society for Biochemistry and Molecular Biology Inc. 2020
• Subjects: Cell culture; Characterization; Desorption; Diseases; Drug products; Epitopes; Mass spectrometry; Monoclonal antibodies; Stem cells; Breast cancer cells; Glycan microarrays; High sensitivity; Human embryonic stem cells (hESC); Monoclonal antibodies (mAb); N-linked glycans; Preclinical development; Structural characterization; Polysaccharides; epitope; glycan; monoclonal antibody; monoclonal antibody A4; oncofetal antigen; small interfering RNA; trisaccharide; unclassified drug; aminosugar; cancer antibody; N-acetyllactosamine; tumor antigen; animal cell; antigen recognition; antineoplastic activity; Article; breast cancer cell line; cell viability; controlled study; drug binding; drug cytotoxicity; drug mechanism; drug targeting; embryo; epitope mapping; female; fucosylation; gene expression; human; human cell; human embryonic stem cell; matrix-assisted laser desorption-ionization mass spectrometry; mouse; nonhuman; ovarian cancer cell line; ovary cancer; protein microarray; breast tumor; cancer stem cell; immunology; ovary tumor; tumor cell line; Amino Sugars; Antibodies, Monoclonal, Murine-Derived; Antibodies, Neoplasm; Antigens, Neoplasm; Breast Neoplasms; Cell Line, Tumor; Female; Humans; Neoplastic Stem Cells; Ovarian Neoplasms
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/179207
• Institution: National University of Singapore

10.1074/jbc.M116.768887