Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications

• Main Authors: Camattari, A, Goh, A, Yip, L.Y, Meng Tan, A.H, Ng, S.W, Tran, A, Liu, G, Liachko, I, Dunham, M.J, Rancati, G
• Other Authors: DEPT OF BIOCHEMISTRY
• Format: Article
• Published: 2020
• Subjects: blue fluorescent protein; fluorescent dye; genomic DNA; recombinant protein; unclassified drug; methanol; Article; controlled study; DNA extraction; expression vector; fluorescence; fluorescence activated cell sorting; fungal genome; fungal strain; gene dosage; genetic recombination; genetic transformation; Komagataella pastoris; molecular cloning; nonhuman; panARS based episomal vector; plasmid; polymerase chain reaction; reporter gene; synthetic biology; yeast cell; biosynthesis; gene expression regulation; gene vector; genetics; metabolism; Pichia; procedures; promoter region; protein processing; Cloning, Molecular; Gene Expression Regulation, Fungal; Genetic Vectors; Methanol; Plasmids; Promoter Regions, Genetic; Protein Processing, Post-Translational; Recombinant Proteins; Recombination, Genetic; Synthetic Biology
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/181404
• Institution: National University of Singapore

10.1186/s12934-016-0540-5